Genome rearrangements in Azospirillum brasilense Sp7 with the involvement of the plasmid pRhico and the prophage ΦAb-Cd

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• 作者：E. I. Katsy ; L. P. Petrova
• 关键词：alphaproteobacteria ; phenotypic variation ; genome instability ; plasmid ; prophage
• 刊名：Russian Journal of Genetics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：51
• 期：12
• 页码：1165-1171
• 全文大小：432 KB
• 参考文献：1.D?bereiner, J. and Day, J.M., Associative symbiosis in tropical grass: characterization of microorganisms and dinitrogen fixing sites, in Symposium on Nitrogen Fixation, Newton, W.E. and Nijmans, C.J., Eds., Pullman: Washington State Univ. Press, 1976, pp. 518-38.
  2.Tarrand, J.X., Krieg, N.E., and D?bereiner, J., A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov., Can. J. Microbiol., 1978, vol. 24, no. 8, pp. 967-80. doi 10.1139/m78-160CrossRef PubMed
  3.Katsy, E.I., Plasticity in Plant-Growth-Promoting and Phytopathogenic Bacteria, New York: Springer, 2014. doi 10.1007/978-1-4614-9203-0CrossRef
  4.Katsy, E.I., Borisov, I.V., Petrova, L.P., and Matora, L.Yu., The use of fragments of the 85and 120-MDa plasmids of Azospirillum brasilense Sp245 to study the plasmid rearrangement in this bacterium and to search for homologous sequences in plasmids of Azospirillum brasilense Sp7, Russ. J. Genet., 2002, vol. 38, no. 2, pp. 124-31. doi 10.1023/A:1009081603530CrossRef
  5.Petrova, L.P., Borisov, I.V., and Katsy, E.I., Plasmid rearrangements in Azospirillum brasilense, Microbiology (Moscow), 2005, vol. 74, no. 4, pp. 495-97. doi 10.1007/s11021-005-0094-8CrossRef
  6.Petrova, L.P., Matora, L.Yu., Burygin, G.L., et al., Analysis of DNA, lipopolysaccharide structure, and some cultural and morphological properties in closely related strains of Azospirillum brasilense, Microbiology (Moscow), 2005, vol. 74, no. 2, pp. 188-93. doi 10.1007/S11021-005-0050-7CrossRef
  7.Petrova, L.P., Shelud’ko, A.V., and Katsy, E.I., Plasmid rearrangements and alterations in Azospirillum brasilense biofilm formation, Microbiology (Moscow), 2010, vol. 79, no. 1, pp. 121-24. doi 10.1134/ S00226261710010169CrossRef
  8.Vial, L., Lavire, C., Mavingui, P., et al., Phase variation and genomic architecture changes in Azospirillum, J. Bacteriol., 2006, vol. 188, no. 15, pp. 5364-373. doi 10.1128/JB.00521-06PubMedCentral CrossRef PubMed
  9.Onyeocha, I., Vieille, C., Zimmer, W., et al., Physical map and properties of a 90-MDa plasmid of Azospirillum brasilense Sp7, Plasmid, 1990, vol. 23, no. 3, pp. 169-82.CrossRef PubMed
  10.Vanbleu, E., Marchal, K., Lambrecht, M., et al., Annotation of the pRhico plasmid of Azospirillum brasilense reveals its role in determining the outer surface composition, FEMS Microbiol. Lett., 2004, vol. 232, no. 2, pp. 165-72. doi 10.1016/S03781097(04)00046-1CrossRef PubMed
  11.Boyer, M., Haurat, J., Samain, S., et al., Bacteriophage prevalence in the genus Azospirillum and analysis of the first genome sequence of an Azospirillum brasilense integrative phage, Appl. Environ. Microbiol., 2008, vol. 74, no. 3, pp. 861-74. doi 10.1128/AEM.02099-07PubMedCentral CrossRef PubMed
  12.Eskew, D.L., Focht, D.D., and Ting, I.P., Nitrogen fixation, denitrification, and pleomorphic growth in a highly pigmented Spirillum lipoferum, Appl. Environ. Microbiol., 1977, vol. 34, no. 5, pp. 582-85.PubMedCentral PubMed
  13.Matveev, V.Yu., Petrova, L.P., Zhuravleva, E.A., and Panasenko, V.I., Characteristics of dissociation in cultures of Azospirillum brasilense Sp7, Mol. Genet. Microbiol. Virusol., 1987, no. 8, pp. 16-8.
  14.Matora, L.Yu., Serebrennikova, O.B., Petrova, L.P., et al., Atypical R–S dissociation in Azospirillum brasilense, Microbiology (Moscow), 2003, vol. 72, no. 1, pp. 48-1. doi 10.1023/A:1022273922287CrossRef
  15.Matora, L.Yu., Burygin, G.L., Shchegolev, S.Yu., Study of immunochemical heterogeneity of Azospirillum brasilense lipopolysaccharides, Microbiology (Moscow), 2008, vol. 77, no. 2, pp. 166-70. doi 10.1134/ S0026261708020070CrossRef
  16.Sigida, E.N., Fedonenko, Y.P., Shashkov, A.S., et al., Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7, Carbohydr. Res., 2013, vol. 380, pp. 76-0. doi 10.1016/jcarres.2013.07.013CrossRef PubMed
  17.Sigida, E.N., The structural heterogeneity of lipopolysaccharides in bacteria of the genus Azospirillum, serogroup II, Extended Abstract of Cand. Sci. Dissertation, Institut Biokhimii i Fiziologii Rastenii i Mikroorganizmov Rossiiskoi Akademii Nauk, Saratov, 2014.
  18.Lerner, A., Valverde, A., Castro-Sowinski, S., et al., Phenotypic variation in Azospirillum brasilense exposed to starvation, Environ. Microbiol. Rep., 2010, vol. 2, no. 4, pp. 577-86. doi 10.1111/j.1758-2229.2010.00149xCrossRef PubMed
  19.Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Lab. Press, 1989, 2nd ed.
  20.NCBI Resource Coordinators, Database resources of the National Center for Biotechnology Information, Nucleic Acids Res., 2014, vol. 42, pp. D7–D17. doi 10.1093/nar/gks1189
  21.Sadasivan, L. and Neyra, C.A., Cyst production and brown pigment formation in a
• 作者单位：E. I. Katsy (1)
  L. P. Petrova (1)
  
  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), Saratov, 410049, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Animal Genetics and Genomics
  Microbial Genetics and Genomics
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3369

文摘

Alphaproteobacteria of the species Azospirillum brasilense have a multicomponent genome that undergoes frequent spontaneous rearrangements, yielding changes in the plasmid profiles of strains. Specifically, variants (Cd, Sp7.K2, Sp7.1, Sp7.4, Sp7.8, etc.) of the type strain A. brasilense Sp7 that had lost a 115-MDa plasmid were previously selected. In many of them, the molecular weight of a 90-MDa plasmid (p90 or pRhico), which is a kind of “depot-for glycopolymer biosynthesis genes, increased. In this study, a collection of primers was designed to the plasmid pRhico and to the DNA of prophage ΦAb-Cd integrated in it. The use of these primers in polymerase chain reactions allowed the detection of the probable excision of ΦAb-Cd phage from the DNA of A. brasilense variants Sp7.4 and Sp7.8 and other alterations of the pRhico structure in A. brasilense strains Cd, Sp7.K2, and Sp7.8. The developed primers and PCR conditions may be recommended for primary analysis of spontaneous plasmid rearrangements in A. brasilense Sp7 and related strains. Keywords alphaproteobacteria phenotypic variation genome instability plasmid prophage