Spatially and temporally restricted expression of PtrMYB021 regulates secondary cell wall formation in Arabidopsis

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• 作者：Wei Wang ; Eryang Li ; Ilga Porth ; Jin-Gui Chen…
• 关键词：Arabidopsis thaliana ; Populus trichocarpa ; PtrMYB021 ; R2R3 MYB ; Secondary cell wall biosynthesis ; Transcription factor
• 刊名：Journal of Plant Biology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：59
• 期：1
• 页码：16-23
• 全文大小：1,972 KB
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• 作者单位：Wei Wang (1)
  Eryang Li (2)
  Ilga Porth (3)
  Jin-Gui Chen (4)
  Shawn D. Mansfield (3)
  Carl J. Douglas (2)
  Shucai Wang (1)
  
  1. Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun, 130024, China
  2. Department of Botany, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada
  3. Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada
  4. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
  
• 刊物主题：Plant Sciences; Plant Breeding/Biotechnology; Plant Genetics & Genomics; Plant Systematics/Taxonomy/Biogeography; Plant Ecology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1867-0725

文摘

Among the R2R3 MYB transcription factors that involve in the regulation of secondary cell wall formation in Arabidopsis, MYB46 alone is sufficient to induce the entire secondary cell wall biosynthesis program. PtrMYB021, the poplar homolog of MYB46, has been reported to regulate secondary cell wall formation when expressed in Arabidopsis. We report here that spatially and temporally restricted expression of PtrMYB021 is critical for its function in regulating secondary cell wall formation. By using quantitative RT-PCR, we found that PtrMYB021 was expressed primarily in xylem tissues. When expressed in Arabidopsis under the control of PtrCesA8, but not the 35S promoter, PtrMYB021 increased secondary cell wall thickness, which is likely caused by increased lignification as well as changes in cell wall carbohydrate composition. In consistent with this, elevated expression of lignin and cellulose biosynthetic genes were observed in the transgenic plants. When expressed in Arabidopsis protoplasts as fusion proteins to the Gal4 DNA binding domain, PtrMYB021 activated the reporter gene Gal4-GUS. In summary, our results suggest that PtrMYB021 is a transcriptional activator, and spatially and temporally restricted expression of PtrMYB021 in Arabidopsis regulates secondary cell wall formation by activating a subset of secondary cell wall biosynthesis genes. Key words Arabidopsis thaliana Populus trichocarpa PtrMYB021 R2R3 MYB Secondary cell wall biosynthesis Transcription factor