Overexpression of a poplar two-pore K+ channel enhances salinity tolerance in tobacco cells
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- 作者：Feifei Wang (1)
Shurong Deng (1)
Mingquan Ding (1) (2)
Jian Sun (1) (3)
Meijuan Wang (1)
Huipeng Zhu (1)
Yansha Han (1)
Zedan Shen (1)
Xiaoshu Jing (1)
Fan Zhang (1)
Yue Hu (1)
Xin Shen (1)
Shaoliang Chen (1) - 关键词：K+ flux ; NaCl ; PeTPK1 ; Populus euphratica ; Protoplast ; Tobacco BY ; 2 cells
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：2013
- 出版时间：January 2013
- 年：2013
- 卷：112
- 期：1
- 页码：19-31
- 全文大小：1128KB
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Shurong Deng (1)
Mingquan Ding (1) (2)
Jian Sun (1) (3)
Meijuan Wang (1)
Huipeng Zhu (1)
Yansha Han (1)
Zedan Shen (1)
Xiaoshu Jing (1)
Fan Zhang (1)
Yue Hu (1)
Xin Shen (1)
Shaoliang Chen (1)
1. College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, 100083, People’s Republic of China
2. College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Hangzhou, 311300, Zhejiang Province, People’s Republic of China
3. College of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu Province, People’s Republic of China - ISSN：1573-5044
文摘
Populus euphratica is a plant model intensively studied for elucidating physiological and molecular mechanisms of salt tolerance in woody species. Several studies have shown that vacuolar potassium (K+) ion channels of the two-pore K+ (TPK) family play an important role in maintaining K+ homeostasis. Here, we cloned a putative TPK channel gene from P. euphratica, termed PeTPK. Sequence analysis of PeTPK1 identified the universal K-channel-specific pore signature, TXGYGD. Over-expression of PeTPK1 in tobacco BY-2 cells improved salt tolerance, but did not enhance tolerance to hyperosmotic stress caused by mannitol (200-00?mM). After 3?weeks of NaCl stress (100 and 150?mM), PeTPK1-transgenic cells had higher fresh and dry weights than wild-type cells. Salt treatment caused significantly higher Na+ accumulation and K+ loss in wild-type cells compared to transgenic cells. During short-term salt stress (100?mM NaCl, 24-h), PeTPK1-transgenic cells showed higher cell viability and reduced membrane permeabilization compared to wild-type cells. Scanning ion-selective electrode data revealed that salt-shock elicited a significantly higher transient K+ efflux from PeTPK1-transgenic callus cells and protoplasts compared to that observed in wild-type cells and protoplasts. We concluded that salt tolerance in P. euphratica is most likely mediated through PeTPK1. We propose that, under salt stress, PeTPK1 functions as an outward-rectifying, K+ efflux channel in the vacuole that transfers K+ to the cytosol to maintain K+ homeostasis.