Functional P2X7 receptors at cultured hippocampal astrocytes but not neurons
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- 作者：Patrizia Rubini ; Gregor Pagel ; Soghra Mehri…
- 关键词：Astrocytes ; Neurons ; Hippocampus ; P2X7 receptors ; Ca2+ imaging ; Patch ; clamp recording
- 刊名：Naunyn-Schmiedeberg's Archives of Pharmacology
- 出版年：2014
- 出版时间：October 2014
- 年：2014
- 卷：387
- 期：10
- 页码：943-954
- 全文大小：1,968 KB
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Gregor Pagel (1)
Soghra Mehri (1)
Peter Marquardt (1)
Thomas Riedel (1)
Peter Illes (1)
1. Rudolf-Boehm-Institute of Pharmacology and Toxicology, University of Leipzig, Haertelstrasse 16-18, 04107, Leipzig, Germany - ISSN：1432-1912
文摘
P2X7 receptors have been suggested to be located both on neurons and astrocytes of the central and peripheral nervous systems. In the present Ca2+-imaging and patch-clamp study, we reinvestigated these findings on mixed neuronal–astrocytic cell cultures prepared from embryonic or newborn rat hippocampi. We found in a Mg2+-free bath medium that the prototypic P2X7 receptor agonist dibenzoyl-adenosine triphosphate (Bz-ATP) increased the intracellular Ca2+ concentration ([Ca2+]i) both in the neuronal cell bodies and in their axo-dendritic processes only to a very minor extent. However, Bz-ATP produced marked [Ca2+]i transients in the neuronal processes, when they grew above a glial carpet, which was uniformly sensitive to Bz-ATP. These glial signals might be misinterpreted as neuronal responses because of the poor focal discrimination by a fluorescent microscope. Most astrocytes had a polygonal shape without clearly circumscribable boundaries, but a subgroup of them had neuron-like appearance. The cellular processes of this astrocytic subgroup, just as their cell somata and their polygonal counterparts, appeared to possess a high density of functional P2X7 receptors. In contrast to astrocytes, in a low Ca2+/no Mg2+-containing bath medium, hippocampal neurons failed to respond to Bz-ATP with membrane currents. In addition, neither the amplitude nor the frequency of spontaneous excitatory postsynaptic currents, representing the quantal release of glutamate, was modified by Bz-ATP. We conclude that cultured hippocampal neurons, in contrast to astrocytes, possess P2X7 receptors, if at all, only at a low density.