A single-neuron tracing study of arkypallidal and prototypic neurons in healthy rats

详细信息    查看全文

• 作者：Fumino Fujiyama ; Takashi Nakano ; Wakoto Matsuda…
• 关键词：Basal ganglia ; Single neuron tracing ; Globus pallidus ; Striatum ; Arkypallidal neuron ; Prototypic neuron
• 刊名：Brain Structure and Function
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：221
• 期：9
• 页码：4733-4740
• 全文大小：1,749 KB
• 刊物主题：Neurosciences; Cell Biology; Neurology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1863-2661
• 卷排序：221

文摘

The external globus pallidus (GP) is known as a relay nucleus of the indirect pathway of the basal ganglia. Recent studies in dopamine-depleted and healthy rats indicate that the GP comprises two main types of pallidofugal neurons: the so-called “prototypic” and “arkypallidal” neurons. However, the reconstruction of complete arkypallidal neurons in healthy rats has not been reported. Here we visualized the entire axonal arborization of four single arkypallidal neurons and six single prototypic neurons in rat brain using labeling with a viral vector expressing membrane-targeted green fluorescent protein and examined the distribution of axon boutons in the target nuclei. Results revealed that not only the arkypallidal neurons but nearly all of the prototypic neurons projected to the striatum with numerous axon varicosities. Thus, the striatum is a major target nucleus for pallidal neurons. Arkypallidal and prototypic GP neurons located in the calbindin-positive and calbindin-negative regions mainly projected to the corresponding positive and negative regions in the striatum. Because the GP and striatum calbindin staining patterns reflect the topographic organization of the striatopallidal projection, the striatal neurons in the sensorimotor and associative regions constitute the reciprocal connection with the GP neurons in the corresponding regions.