Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation

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• 作者：Liang Yang (1) (2)
  Zhenguo Du (1) (4)
  Feng Gao (3)
  Kangcheng Wu (1) (2)
  Lianhui Xie (1) (2)
  Yi Li (3)
  Zujian Wu (1) (2)
  Jianguo Wu (1) (2) (3)
  
  1. Key Laboratory of Plant Virology of Fujian Province ; Institute of Plant Virology ; Fujian Agriculture and Forestry University ; Fuzhou ; Fujian ; 350002 ; China
  2. Key Laboratory of Biopesticide and Chemibiology of Ministry of Education ; Fujian Agriculture and Forestry University ; Fuzhou ; Fujian ; 350002 ; China
  4. Guangdong Provincial Key Laboratory of High Technology for Plant Protection ; Guangzhou ; 510640 ; China
  3. Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology ; The State Key Laboratory of Protein and Plant Gene Research ; College of Life Sciences ; Peking University ; Beijing ; 100871 ; China
  
• 关键词：RDV ; Transcriptome profiling ; Pns11 ; Nucleolus
• 刊名：Virology Journal
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：11
• 期：1
• 全文大小：756 KB
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• 刊物主题：Virology;
• 出版者：BioMed Central
• ISSN：1743-422X

文摘

Background Rice dwarf virus (RDV) is the causal agent of rice dwarf disease, which limits rice production in many areas of south East Asia. Transcriptional changes of rice in response to RDV infection have been characterized by Shimizu et al. and Satoh et al.. Both studies found induction of defense related genes and correlations between transcriptional changes and symptom development in RDV-infected rice. However, the same rice cultivar, namely Nipponbare belonging to the Japonic subspecies of rice was used in both studies. Methods Gene expression changes of the indica subspecies of rice, namely Oryza sativa L. ssp. indica cv Yixiang2292 that show moderate resistance to RDV, in response to RDV infection were characterized using an Affymetrix Rice Genome Array. Differentially expressed genes (DEGs) were classified according to their Gene Ontology (GO) annotation. The effects of transient expression of Pns11 in Nicotiana benthaminana on the expression of nucleolar genes were studied using real-time PCR (RT-PCR). Results 856 genes involved in defense or other physiological processes were identified to be DEGs, most of which showed up-regulation. Ribosome- and nucleolus related genes were significantly enriched in the DEGs. Representative genes related to nucleolar function exhibited altered expression in N. benthaminana plants transiently expressing Pns11 of RDV. Conclusions Induction of defense related genes is common for rice infected with RDV. There is a co-relation between symptom severity and transcriptional alteration in RDV infected rice. Besides ribosome, RDV may also target nucleolus to manipulate the translation machinery of rice. Given the tight links between nucleolus and ribosome, it is intriguing to speculate that RDV may enhance expression of ribosomal genes by targeting nucleolus through Pns11.