Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

详细信息    查看全文

• 作者：Agata Ptak (1160)
  Anna El Tahchy (2160)
  Edyta Skrzypek (3160)
  Tomasz Wójtowicz (1160)
  Dominique Laurain-Mattar (2160)
  
• 关键词：Somatic embryos ; 2 ; 4 ; D ; Dicamba ; Picloram ; Alkaloids ; Phenolic compounds
• 刊名：Central European Journal of Biology
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：8
• 期：6
• 页码：591-599
• 全文大小：657KB
• 参考文献：1. Luttmann E., Linnemann E., Fels G., Galanthamine, as bis-functional ligand for the acetylcholinesterase, J. Mol. Model., 2002, 8, 208-16 CrossRef
  2. Heinrich M., Lee Teoh H., Galanthamine from snowdrop-the development of a modern drug against Alzheimer’s disease from local Caucasian knowledge, J. Ethnopharmacol., 2004, 92, 147-2 j.jep.2004.02.012">CrossRef
  3. Szlavik L., Gyuris A., Forgo P., Molnar J., Hohmann J., Alkaloids from Leucojum vernum and antiretroviral activity of Amaryllidaceae alkaloids, Planta Medica, 2004, 70, 871-73 CrossRef
  4. Berkov S., Georgieva L., Kondakova V., Atanassov A., Viladomat F., Bastida J., et al., Plant sources of galanthamine: phytochemical and biotechnological aspects, Biotechnol.&Biotechnol. EQ, 2009, 23, 1170-176
  5. Guillou C., Beunard J.L., Gras E., Thal C., An efficient total synthesis of (±)-galanthamine, Angew. Chem. Int., 2001, 40, 4745-746 CrossRef
  6. Berkov S., Pavlov A., Illieva M., Burrus M., Popov S., Stanilova M., CGC-MS of alkaloids in Leucojum aestivum plants and their in vitro cultures, Phytochem. Anal., 2005, 16, 98-03 CrossRef
  7. Magnus P., Sane N., Fauber B.P., Lynch V., Concise syntheses of (?-galanthamine and (±)-codeine via intramolecular alkylation of a phenol derivative, J. Am. Chem. Soc., 2009, 131, 16045-6047 ja9085534">CrossRef
  8. Briskin D.P., Biotechnological methods for selection of high-yielding cell lines and production of secondary metabolites in medicinal plants, In: Kayser O., Quax W. (Eds.), Medicinal Plant Biotechnology. From Basic Research to Indrustrial Applications, vol. 1, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, 2007
  9. Diop M.F., Ptak A., Chretien F., Henry M., Chapleur Y., Laurain-Mattar D., Galanthamine content of bulbs and in vitro cultures of Leucojum aestivum L., Nat. Prod. Com., 2006, 1, 475-79
  10. Pavlov A., Berkov S., Courot E., Gocheva T., Tuneva D., Pandova B., et al., Galanthamine production by Leucojum aestivum in vitro systems, Process Biochemistry, 2007, 42, 734-39 j.procbio.2006.12.006">CrossRef
  11. Ptak A., El Tahchy A., Dupire F., Boisbrun M., Henry M., Chapleur Y., et al., LCMS and GC-MS for the screening of alakaloids in natural and in vitro extracts of Leucojum aestivum, J. Nat. Prod., 2009, 72, 142-47 CrossRef
  12. Ptak A., El Tahchy A., Wy?golik G., Henry M., Laurain-Mattar D., Effects of ethylene on somatic embryogenesis and galanthamine content in Leucojum aestivum L. cultures, Plant Cell Tiss. Org. Cult., 2010, 102, 61-7 CrossRef
  13. Stanilova M.I., Molle E.D., Yanev S.G., Galanthamine production by Leucojum aestivum cultures in vitro, In: Cordell A (Ed.), The Alkaloids. Chemistry and Biology, vol 68, Elsevier, Amsterdam, Boston, Heidelberg, London, New York, Oxford Paris, San Diego, San Francisco, Sydney, Tokyo, 2010
  14. Ivanov I., Georgiev V., Georgiev M., Ilieva M., Pavlov A., Galanthamine and related alkaloids production by Leucojum aestivum L. shoot culture using a temporary immersion technology, Appl. Biochem. Biotechnol., 2011, 163, 268-77 CrossRef
  15. Ivanov, I., Georgiev, V., Berkov S., Pavlov A., Alkaloid patterns in Leucojum aestivum shoot culture cultivated at temporary immersion conditions, J. Plant Physiol., 2012, 169, 206-11 j.jplph.2011.09.010">CrossRef
  16. El Tahchy A., Bordage S., Ptak A., Dupire F., Barre E., Guillot C., et al., Effects of sucrose and plant growth regulators on acetylcholinesterase inhibitory activity of alkaloids accumulated in shoot cultures of Amaryllidaceae, Plant Cell Tiss. Organ Cult., 2011, 106, 381-90 CrossRef
  17. Laurain-Mattar D., Production of alkaloids in plant cell and tissue cultures, In: Ramawat K.G., Mérillon J.M. (Eds.), Bioactive Molecules and Medicinal Plants, Springer-Verlag, Berlin, Heidelberg, 2008
  18. Jiménez V.M., Thomas C., Participation of plant hormones in determination and progression of somatic embryogenesis, In: Mujib A., ?amaj J. (Eds.), Plant Cell Monographs. Somatic Embryogenesis, 2, Springer, Berlin, 2006
  19. Von Arnold S., Somatic embryogenesis, In: George E.F., Hall M.A., de Klerk G.J. (Eds.), Plant Propagation by Tissue Culture, 3rd Edition, Springer, The Netherlands, 2008
  20. Filipov M., Miroshnichenko D., Vernikovskaya D., Dolgov S., The effect of auxins, time exposure to auxin and genotypes on somatic embryogenesis from mature embryos of wheat, Plant Cell Tissue Organ Cult., 2006, 84, 213-22
  21. Bach A. Interaction between type of tissue and growth regulators in somatic embryogenesis of hyacinth, Acta Physiol. Plant., 1998, 20, 15 CrossRef
  22. Lu W., Enomoto K., Fukunaga Y., Kuo C., Regeneration of petals, stamens and ovules in explants from perianth of Hyacinthus orientalis L. Importance of explant age and exogenous hormones, Planta, 1988, 175, 478-84 CrossRef
  23. Ptak A., Bach A., Somatic embryogenesis in tulip (Tulipa gesneriana L.) flower stem cultures, In Vitro Cell. Dev. Biol. Plant, 2007, 43, 35-9 CrossRef
  24. Tribulato A., Remotti P.C., Loffler H.J.M., Somatic embryogenesis and plant regeneration in Lilium longiflorum Thunb., Plant Cell Rep., 1997, 17, 113-18 CrossRef
  25. Murphy A., Peer W.A., Taiz L., Regulation of auxin transport by aminopeptidases and endogenous flavonoids, Planta, 2000, 211, 315-24 CrossRef
  26. Schoenwaelder M.A., Wiencke C., Phenolic compounds in the embryo development of several northern hemisphere fucoids, Plant Biol., 2000, 2, 24-3 CrossRef
  27. Ptak A., Cierniak O., Regeneration of summer snowflake (Leucojum aestivum L.) in in vitro cultures, Biotechnologia, 2003, 4, 239-45
  28. Murashige T., Skoog F., Revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 1962, 15, 473-97 j.1399-3054.1962.tb08052.x">CrossRef
  29. Pathan A.K., Bond J., Gaskin R.E., Sample preparation for scanning electron microscopy of plant surfaces-Horses for courses, Micron, 2008, 39, 1049-061 j.micron.2008.05.006">CrossRef
  30. Singleton V.S., Rossi Jr J.A., Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagent, Amer. J. Enol. Viticult., 1965, 16, 144-58
  31. Malik M., Molenda A., Formation of narcissus (Narcissus L.) somatic embryos from callus tissue in temporary immersion system with RITA and on solid media, Adv. Agr. Sci. Problem Issue, 2008, 525, 237-43, (in Polish)
  32. Sage D., Lynn J., Hammatt N., Somatic embryogenesis in Narcissus pseudonarcissus cvs. Golden Harvest and St. Keverne, Plant Sci., 2000, 2, 209-16 CrossRef
  33. Zhang T., Cao Z.Y., Wang X.Y., Induction of somatic embryogenesis and plant regeneration from cotyledon and hypocotyls explants of Eruca sativa Mill., In Vitro Cell. Dev. Biol. Plant, 2005, 41, 655-57 CrossRef
  34. Moon H.K., Park S.Y., Kim Y.W., Kim S.H., Somatic embryogenesis and plantlet production using rejuvenated tissues from serial grafting of a mature Kalopanax septemlobus tree, In Vitro Cell. Dev. Biol. Plant, 2008, 44, 119-27 CrossRef
  35. Ptak A., Somatic embryogenesis in in vitro culture of Leucojum vernum L., In: Jain S.M., Ochatt S.J. (Eds.), Protocols for In Vitro Propagation of Ornamental Plants, Springer, New York, Dordrecht, Heidelberg, London, 2010
  36. Stasolla C., Zyl L., Egertsdotter U., Craig D., Liu W., Sederoff R.R., The effects of polyethylene glycol on gene expression of developing white spruce somatic embryos, Plant Physiol., 2003, 131, 49-0 CrossRef
  37. Tanaka N., Shimomura K., Ishimaru K., Tanin production in callus cultures of Quercus acutissima, Phytochem., 1995, 40, 1151-154 CrossRef
  38. Close C.D., Davies N.W., Beadle Ch.L., Temporal variation of tannins (galloylglucoses), flavonols and anthocyanins in leaves of Eucalyptus nitens seedlings: implications for light attenuation and antioxidant activities, Aust. J. Plant Physiol., 2001, 28, 269-78
  39. Skrzypek E., Szechynska-Hebda M., D?browska G., The influence of phenolics accumulation on callus regeneration abilities of chosen plant species, Adv. Agr. Sci. Problem Issue, 2007, 523, 203-12, (in Polish)
  40. Bach A., Paw’owska B., Hura K., The level of phenolic compounds at various developmental stages in in vitro cultures of Galanthus elwesii Hook., Adv. Agr. Sci. Problem Issue, 2009, 534, 13-1, (in Polish)
  41. Hrubcová M., Cvirková M., Eder J., Peroxidase activities and contents of phenolic acids in embryogenic and nonembryogenic alfalfa cell suspension cultures, Biol. Plant., 1994, 36, 75-82 CrossRef
  42. Cvirková M., Binarová P., Eder J., Vágner M., Hrubcová M., Zoń J., et al., Effect of inhibition of phenylanine ammonia-lyase activity on growth of alfalfa cell suspensions culture: alterations in mitotic index, ethylene production, and contents of phenolics, cytokinins and poliamines, Physiol. Plant., 1999, 107, 329-37 j.1399-3054.1999.100310.x">CrossRef
  43. Beruto M., Cuiri P., Debergh P., Callus growth and somatic embryogenesis in thalamus tissue of Ranunculus asiaticus L. cultivated in vitro: cytokinin effect and phenol metabolism, In Vitro Cell. Dev. Biol. Plant, 1996, 32, 154-60 CrossRef
  44. Codina C., Narcissus and Daffodil. The genus Narcissus, In: Hanks G. (Ed.), Medicinal and Aromatic Plants -Industrial Profiles, Taylor and Francis, London, New York, 2002
  45. Ptak A., Simlat M., Kwiecień M., Laurain-Mattar D., Leucojum aestivum plants propagated in in vitro bioreactor culture and on solid media containing cytokinins, Eng. Life Sci., (in press), DOI: 10.1002/elsc.201200109
  46. Khan T., Krupadanam D., Anwar S.Y., The role of phytohormone on the production of berberine in the calli cultures of an endangered medicinal plant, turmeric (Coscinium fenestratum I.), Afr. J. Biotech., 2008, 7, 3244-246
  
• 作者单位：Agata Ptak (1160)
  Anna El Tahchy (2160)
  Edyta Skrzypek (3160)
  Tomasz Wójtowicz (1160)
  Dominique Laurain-Mattar (2160)
  
  1160. Department of Plant Breeding and Seed Science, Agricultural University, 31-140, Krakow, Poland
  2160. Groupe SUCRES, UMR 7565 CNRS, Université de Lorraine, BP 239, 54506, Nancy-Vandoeuvre, France
  3160. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 30-239, Krakow, Poland
  
• ISSN：1644-3632

文摘

In vitro cultures of Leucojum aestivum are considered as an alternative for the production of galanthamine, which is used for the symptomatic treatment of Alzheimer’s disease. We studied the effects of auxins 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,6-dichloro-o-anisic acid (dicamba) at concentrations of 25 and 50 μM on the induction of embryogenic callus and its capacity to induce somatic embryogenesis and alkaloid accumulation. The embryogenic response of the explants was from 30% for 25 μM of dicamba to 100% for picloram (for both 25 and 50 μM). 2,4-D (50 μM) stimulated greater callus proliferation and somatic embryo induction as compared to the other auxins. Polyethylene glycol (PEG) stimulated somatic embryo maturation. Callus grown on media containing 50 μM of auxins produced fewer phenolic compounds as compared with callus grown on media containing 25 μM of auxins. GC-MS analyses showed seven alkaloids in the in vivo bulbs and two to four in callus culture. Galanthamine was detected in callus cultivated with 2,4-D (25, 50 μM), picloram (25 μM), and dicamba (50 μM). Other alkaloids, trisphaeridine, tazettine, and 11-hydroxyvittatine were accumulated only in callus growing on medium with picloram (50 μM).