The effect of Tween 80 on the polymalic acid and pullulan production by Aureobasidium pullulans CCTCC M2012223

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• 作者：Guangwei Tu ; Yongkang Wang ; Yunchao Ji…
• 关键词：Aureobasidium pullulans ; Gene transcription ; Polymalic acid ; Pullulan ; Tween 80
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：31
• 期：1
• 页码：219-226
• 全文大小：1,477 KB
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  5. Duan X, Chi Z, Wang L, Wang X (2008) Influence of different sugars on pullulan production and activities of α-phosphoglucose mutase, UDPG-pyrophosphorylase and glucosyltransferase involved in pullulan synthesis in / Aureobasidium pullulans Y68. Carbohydr Polym 73(4):587-93 CrossRef
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• 作者单位：Guangwei Tu (1) (2)
  Yongkang Wang (1) (2)
  Yunchao Ji (3)
  Xiang Zou (1) (2)
  
  1. College of Pharmaceutical Sciences, Southwest University, 2 Tian Sheng Road, Beibei, Chongqing, 400715, People’s Republic of China
  2. Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control, Chongqing, 400715, People’s Republic of China
  3. PKU Care Daxin Pharmaceuticals Co., Ltd, Chongqing, 400715, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

The effect of Tween 80 on the fermentative production of polymalic acid (PMA) and pullulan using Aureobasidium pullulans CCTCC M2012223 was investigated. Tween 80 is beneficial for the biosynthesis of PMA and pullulan, and can regulate the ratio of PMA to pullulan in a dose-dependent manner. After adding 0.05?% Tween 80 to the media, the maximal PMA and pullulan production was 46.45 and 28.8?g/L at 60?h in a 5?L fermenter, with an increase of 75.08 and 27.21?% when compared to the control. Tween 80 could regulate and enhance oxygen uptake rate and carbon dioxide evolution rate in the early phase of fermentation, and change the cell morphology. The transcription levels of mitochondrial dicarboxylate transporter and transmembrane transporter were also dramatically upregulated. The present work will be helpful in deeply understanding the mechanism of Tween 80 on the effect of PMA and pullulan production.