Vitamin E synthetic derivate—TPGS—selectively induces apoptosis in jurkat t cells via oxidative stress signaling pathways: implications for acute lymphoblastic leukemia

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• 作者：Cristian Ruiz-Moreno ; Marlene Jimenez-Del-Rio ; Ligia Sierra-Garcia…
• 刊名：Apoptosis
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：21
• 期：9
• 页码：1019-1032
• 全文大小：3,391 KB
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Cancer Research
  Cell Biology
  Biochemistry
  Virology
  
• 出版者：Springer Netherlands
• ISSN：1573-675X
• 卷排序：21

文摘

D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) is a water-soluble derivative of natural vitamin E commonly used as a drug delivery agent. Recently, TPGS alone has been reported to induce cell death in lung, breast and prostate cancer. However, the effect of TPGS on cancer cell viability remains unclear. Thus, this study was aimed to evaluate the cytotoxic effect of TPGS on human periphral blood lymphocytes (PBL) and on T cell acute lymphocytic leukemia (ALL) Jurkat clone E6-1 cells and its possible mechanism of action. PBL and Jurkat cells were treated with TPGS (10, 20, 40, 60, and 80 μM), and morphological changes in the cell nucleus, mitochondrial membrane potential (ΔΨ<sub>msub>), and intracellular reactive oxygen species levels were determined by immune-fluorescence microscopy and flow cytometry. Cellular apoptosis markers were also evaluated by immunocytochemistry. In this study, TPGS induced apoptotic cell death in Jurkat cells, but not in PBL, in a dose–response manner with increasing nuclear DNA fragmentation, increasing cell cycle arrest, and decreasing ΔΨ<sub>msub>. Additionally, TPGS increased dichlorofluorescein fluorescence intensity, indicative of H<sub>2sub>O<sub>2sub> production, in a dose-independent fashion. TPGS increased DJ-1 Cys106-sulfonate, as a marker of intracellular stress and induced the activation of NF-κB, p53 and c-Jun transcription factors. Additionally, it increased the expression of apoptotic markers Bcl-2 related pro-apoptotic proteins Bax and PUMAand activated caspase-3. The antioxidant N-acetyl-l-cysteine and known pharmacological inhibitors protected the cells from the TPGS induced effects. In conclusion, TPGS selectively induces apoptosis in Jurkat cells through two independent but complementary H<sub>2sub>O<sub>2sub>-mediated signaling pathways. Our findings support the use of TPGS as a potential treatment for ALL.KeywordsApoptosisJurkatLeukemiaVitamin EAlpha-tocopheryl polyethylene glycol succinate