Effects of low molecular weight hyaluronan combined with carprofen on canine osteoarthritis articular chondrocytes and cartilage explants in vitro

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• 作者：Thippaporn Euppayo ; Puntita Siengdee…
• 关键词：Articular cartilage ; Carprofen ; Cartilage explant ; Hyaluronan ; Osteoarthritis
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：51
• 期：8
• 页码：857-865
• 全文大小：594 KB
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• 作者单位：Thippaporn Euppayo (1)
  Puntita Siengdee (1)
  Kittisak Buddhachat (2)
  Waranee Pradit (2)
  Nawarat Viriyakhasem (3)
  Siriwadee Chomdej (2)
  Siriwan Ongchai (3)
  Yasuji Harada (4)
  Korakot Nganvongpanit (1) (5)
  
  1. Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand
  2. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand
  3. Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand
  4. Divisions of Veterinary Surgery, Nippon Veterinary and Life Science University, 1-7-1, Kyonann-cho, Musashinoshi, Tokyo, 180-8602, Japan
  5. Excellence Center in Osteology Research and Training Center, Chiang Mai University, Chiang Mai, 50200, Thailand
  
• 刊物主题：Cell Biology; Developmental Biology; Stem Cells; Cell Culture; Animal Genetics and Genomics;
• 出版者：Springer US
• ISSN：1543-706X

文摘

Intra-articular injection with non-steroidal anti-inflammatory drugs (NSAIDs) is used to treat inflammatory joint disease, but the side effects of NSAIDs include chondrotoxicity. Hyaluronan has shown positive effects on chondrocytes by reducing apoptosis and increasing proteoglycan synthesis. The purposes of this study were to evaluate the effects of low molecular weight hyaluronan (low MW HA), carprofen 25 mg/ml, carprofen 12.5 mg/ml, and a combination of HA and carprofen on canine osteoarthritis (OA) articular chondrocytes and a cartilage explant model in terms of cell viability, extracellular matrix remaining, and gene expression after exposure. In chondrocyte culture, MTT assay was used to evaluate the chondrotoxicity of IC₅₀ and IC₈₀ of carprofen with HA. In cartilage explant culture, two kinds of extracellular matrix (uronic acid and collagen) remaining in cartilage were used to evaluate cartilage damage for 14 d after treatment. Expression of COL2A1, AGG, and MMP3 was used to evaluate the synthesis and degradation of the matrix for 7 d after treatment. In chondrocyte culture, low MW HA could preserve OA chondrocyte viability but could not reduce the chondrotoxicity level of carprofen (P-lt;-.05). In explant culture, low MW HA combined with 12.5 mg/ml carprofen caused less destruction of uronic acid and collagen structure when compared with the control (P-lt;-.05). Low MW HA caused high expression levels of COL2A1 and AGG in OA cartilage (P-lt;-.05); HA combined with carprofen resulted in higher COL2A1 and AGG expression levels than carprofen alone. Keywords Articular cartilage Carprofen Cartilage explant Hyaluronan Osteoarthritis