Systematic analyses of the ultraviolet radiation resistance-associated gene product (UVRAG) protein interactome by tandem affinity purification

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• 作者：Ji-Hye Son ; Eurim C. Hwang ; Joungmok Kim
• 关键词：UVRAG ; Tandem affinity purification ; Interactome analysis
• 刊名：Archives of Pharmacal Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：39
• 期：3
• 页码：370-379
• 全文大小：845 KB
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• 作者单位：Ji-Hye Son (1)
  Eurim C. Hwang (2)
  Joungmok Kim (1)
  
  1. Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Research Center for Tooth and Periodontal Tissue Regeneration, Kyung Hee University, Seoul, 130-701, Korea
  2. Department of Chemistry, New York University, New York, NY, 10003, USA
  
• 刊物主题：Pharmacy; Pharmacology/Toxicology;
• 出版者：Springer Netherlands
• ISSN：1976-3786

文摘

Ultraviolet radiation resistance-associated gene product (UVRAG) was originally identified as a protein involved in cellular responses to UV irradiation. Subsequent studies have demonstrated that UVRAG plays as an important role in autophagy, a lysosome-dependent catabolic program, as a part of a pro-autophagy PIK3C3/VPS34 lipid kinase complex. Several recent studies have shown that UVRAG is also involved in autophagy-independent cellular functions, such as DNA repair/stability and vesicular trafficking/fusion. Here, we examined the UVRAG protein interactome to obtain information about its functional network. To this end, we screened UVRAG-interacting proteins using a tandem affinity purification method coupled with MALDI-TOF/MS analysis. Our results demonstrate that UVRAG interacts with various proteins involved in a wide spectrum of cellular functions, including genome stability, protein translational elongation, protein localization (trafficking), vacuole organization, transmembrane transport as well as autophagy. Notably, the interactome list of high-confidence UVRAG-interacting proteins is enriched for proteins involved in the regulation of genome stability. Our systematic UVRAG interactome analysis should provide important clues for understanding a variety of UVRAG functions.