Purification and characterization of an alkaline pectin lyase produced by a newly isolated Brevibacillus borstelensis (P35) and its applications in fruit juice and oil extraction

详细信息    查看全文

• 作者：Nazan Demir (1)
  Hayrunnisa Nadaroglu (2)
  Yasar Demir (1) (3)
  Ceyhun Isik (1)
  Esen Taskin (4)
  Ahmet Adiguzel (5)
  Medine Gulluce (6)
  
• 关键词：Pectin lyase ; Purification ; Brevibacillus borstelensis (P35) ; Pectin ; Juice clarification ; Oil extraction
• 刊名：European Food Research and Technology
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：239
• 期：1
• 页码：127-135
• 全文大小：
• 参考文献：1. Serrat M, Bermúdez RC, Villa TG (2002) Production, purification, and characterization of a polygalacturonase from a new strain of / Kluyveromyces marxianus isolated from coffee wet-processing wastewater. Appl Biochem Biotechnol 97:193-08 CrossRef
  2. Yadav S, Yadav PK, Yadav D, Yadav KDS (2008) Purification and characterization of an alkaline extracellular pectin lyase produced by / Aspergillus flavus MTCC 7589. Process Biochem 43:547-52 CrossRef
  3. Alkorta I, Garbisu C, Liama MJ, Serra JL (1998) Industrial application of pectic enzymes: a review. Process Biochem 33:21-8 CrossRef
  4. Demir N, Nadaroglu H, Tasg?n E, Adiguzel A, Gulluce M (2011) Purification and characterization of a pectin lyase produced by / Geobacillus stearothermophilus (Ah22) and fruit juice application. Ann Microbiol 61(4):939-46 CrossRef
  5. Nadaroglu H, Taskin E, Adiguzel A, Gulluce M, Demir N (2010) Production of a pectin lyase from / Bacillus pumilus (P9), purification and characterisation and fruit juice application. Rom Biotechnol Lett 15(2):5167-176
  6. Whitaker JR (1990) Microbial pectolytic enzymes. In: Fogarty WM, Kelly CT (eds) Microbial enzymes and biotechnology, 2nd edn. Elsevier Applied Science, New York, pp 133-76 CrossRef
  7. Maugeri TL, Gugliandolo C, Caccamo D, Stackebrandt E (2001) A polyphasic taxonomic study of thermophilic bacilli from shallow, marine vents. Syst Appl Microbiol 24(4):572-87 CrossRef
  8. Rahman TJ, Marchant R, Banat IM (2004) Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments. Biochem Soc Trans 32(2):209-13 CrossRef
  9. Savas S, Adiguzel A, Inan K, Ozkan H, Gulluce M, Sahin F (2009) Molecular characterization of thermophilic bacteria isolated from Van city Ercis town Hasanabdal hot spring. Rom Biotechnol Lett 14(3):4445-454
  10. Fahy PC, Hayward AC (1983) Plant bacterial diseases: a diagnostic guide. Academic Press, Sydney
  11. Adiguzel A (2006) Molecular characterization of thermophilic bacteria isolated from water samples taken from various thermal plants. PhD thesis, Ataturk University, Erzurum, p 130
  12. Beffa T, Blanc M, Lyon PF, Vogt G, Marchiani M, Fischer JL, Aragno M (1996) Isolation of Thermus strains from hot composts (60 to 80°C). Appl Environ Microbiol 62:1723-727
  13. Adiguzel A, Ozkan H, Baris O, Inan K, Gulluce M (2009) Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey. J Microbiol Methods 79:321-28 CrossRef
  14. Taragano VM, Pilosof AMR (1999) Application of Doehlert designs for water activity, pH, and fermentation time optimization for / Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation. Enzym Microb Technol 25:411-19 CrossRef
  15. Kashyap DR, Chandra S, Kau A, Tewari R (2000) Production, purification and characterization of pectinase from a / Bacillus sp. DT7. World J Microbiol Biotechnol 16:277-82 CrossRef
  16. Nedjma M, Hoffmann N, Belarbi A (2001) Selective and sensitive detection of pectin lyase activity using a colorimetric test: application to the screening of microorganisms possessing pectin lyase activity. Anal Biochem 291:290-96 CrossRef
  17. Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248-53 CrossRef
  18. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 22:680-85 CrossRef
  19. Whitaker JR (1963) Determination of molecular weight of proteins by gel filtration on Sephadex. Anal Chem 35:1950-953 CrossRef
  20. Soraes MMCN, Da Silva R, Carmona EC, Gomes E (2001) Pectinolytic enzyme production by / Bacillus species and their potential application on juice extraction. World J Microbiol Biotechnol 17:79-2 CrossRef
  21. Hadj-Taieb N, Ayadi M, Khlif M, Mrad K, Hassairi I, Gargouri A (2006) Fermentor production of pectinases on gruel, a local by-product and their use in olive oil extraction. Enzym Microb Technol 39(5, 4):1072-076 CrossRef
  22. Perry JJ, Staley JT (1997) Taxonomy of eubacteria and archaea. In: Perry JJ, Staley JT (eds) Microbiology: dynamics and diversity. Saunders College Publishing, Fort Worth, pp 388-13
  23. Martins ES, Silva D, Da Silva R, Gomes E (2002) Solid state production of thermostable pectinases from thermophilic / Thermoascus aurantiacus. Process Biochem 37:949-54 CrossRef
  24. Sinitsyna OA, Fedorova EA, Semenova MV, Gusakov AV, Sokolova LM, Bubnova TM, Okunev ON, Chulkin AM, Vavilova EA, Vinetsky YP, Sinitsyn AP (2007) Isolation and characterization of extracellular pectin lyase from / Penicillium canescens. Biochemistry (Moscow) 72:565-71 CrossRef
  25. Kamimiya S, Nishiya T, Izaki K, Takahashi H (1974) Purification and properties of a pectin trans-eliminase in Erwinia aroideae formed in the presence of nalidixic acid. Agric Biol Chem 38:1071-078 CrossRef
  26. Yadav S, Shastri NV (2007) Purification and properties of an extracellular pectin lyase produced by the strain of / Penicillium oxalicum in solid-state fermentation. Indian J Biochem Biophys 44:247-51
  27. Nakagawa T, Nagaoka T, Miyaji T, Tomizuka N (2005) A cold active pectin lyase from psychrophilic and basidiomycetous yeast Cystofilobasidium capitatum strain PPY-1. Biotechnol Appl Biochem 42:193-96 CrossRef
  28. Chen WC, Hsieh HJ, Tseng TC (1998) Purification and characterization of a pectin lyase from / Pythium splendens infected cucumber fruits. Bot Bull Acad Sin 39:181-86
  29. Cao J, Zheng L, Chen S (1992) Screening of pectinase producer from alkalophilic bacteria and study on its potential application in degumming of ramie. Enzyme Microb Technol 14:1013-016 CrossRef
  30. Bruhlmann F, Leupin M, Erismann KH, Fiechter A (2000) Enzymatic degumming of ramie bast fibers. J Biotechnol 76:43-0 CrossRef
  31. Henriksson G, Akin DE, Rigsby LL, Patel N, Eriksson KEL (1997) Influence of chelating agents and mechanical pretreatment on enzymatic retting of flax. Text Res J 67:829-36
  32. Kapoor M, Beg QK, Bhushan B, Singh K, Dadhich KS, Hoondal GS (2001) Application of alkaline thermostable polygalacturonase from Bacillus sp. MG-ep-2 in degumming of ramie ( / Boehemeria nivea) and sunn hemp ( / Crotalaria juncea) bast fibers. Process Biochem 6:803-07 CrossRef
  33. Nagel CW, Hasegawa S (1968) The isolation and characterization of an 1,4 (4,4-dihydrogalacturonosyl) galacturonate hydrolase. J Food Sci 33:378-82 CrossRef
  34. Starr MP, Nasumo S (1967) Proteolytic activity of phytopathogenic xanthomonads. J Gen Microbiol 46:425-33 CrossRef
  35. Dave BA, Vaughn RH (1971) Purification and properties of a polygalaturonic acid trans eliminase produced by / Bacillus pumilus. J Bacteriol 108:166-74
  36. Horikoshi K (1972) Production of alkaline enzymes by alkalophilic microorganisms. Part III. Alkaline pectinase of Bacillus no. P-4-N. Agric Biol Chem 36:285-93 CrossRef
  37. Kelly CT, Fogarty WM (1978) Production and properties of polygalacturonate lyase by an alkalophilic microorganism / Bacillus sp. RK9. Can J Microbiol 24(10):1164-172 CrossRef
  
• 作者单位：Nazan Demir (1)
  Hayrunnisa Nadaroglu (2)
  Yasar Demir (1) (3)
  Ceyhun Isik (1)
  Esen Taskin (4)
  Ahmet Adiguzel (5)
  Medine Gulluce (6)
  
  1. Department of Chemistry, Faculty of Sciences, Mugla University, 48000, Mugla, Turkey
  2. Department of Food Technology, Erzurum Vocational Training School, Ataturk University, 25240, Erzurum, Turkey
  3. Department of Chemistry, Faculty of Science, University of Mehmet Akif Ersoy, 15000, Burdur, Turkey
  4. Faculty of Education, Elementary Science Education, Bayburt University, 69100, Bayburt, Turkey
  5. Department of Molecular Biology and Genetic, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey
  6. Department of Biology, Faculty of Arts and Sciences, Ataturk University, 25240, Erzurum, Turkey
  
• ISSN：1438-2385

文摘

An alkaline pectin lyase (PNL) (EC 4.2.2.10) secreted by Brevibacillus borstelensis P35 (GenBank Number: FJ417406) was purified using ammonium sulfate fractionation, anion exchange chromatography on DEAE-cellulose and gel filtration chromatography on Sephadex G-150. The pH and temperature optima of the enzyme were found to be 8.0 and 60?°C. The enzyme does not loose activity up to 60?°C if exposed for 1?h. The values of K m and V max of the enzyme were 0.625?mg/mL and 126.32?s?, respectively. The molecular weight was found to be 36?±?01?kDa. The presence of 10?mM concentration of Ca2+, Cu2+, Mn2+, Mg2+, Zn2+, Hg2+, Fe2+ and EDTA, l-cystein, ascorbic acid significantly enhanced the PNL of the purified enzyme. In the course of the laboratory trials, it was demonstrated that PNL from B. borstelensis (P35) could be successfully applied to the production and clarification of fruit juice and oil extraction.