Production and Characterization of Bio-AuNPs to Induce Synergistic Effect Against Multidrug Resistant Bacterial Biofilm

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• 作者：Sekar Vanaraj ; Jayamanohar Jabastin ; Swamiappan Sathiskumar…
• 关键词：Bioreduction ; Gold nanoparticle ; Clitoria ternatea L. ; Antimicrobial activity ; Biofilm hemocompatible
• 刊名：Journal of Cluster Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：28
• 期：1
• 页码：227-244
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Catalysis; Inorganic Chemistry; Physical Chemistry; Nanochemistry;
• 出版者：Springer US
• ISSN：1572-8862
• 卷排序：28

文摘

Herein we demonstrate gold nanoparticles were synthesized by conventional bioreduction approach using Clitoria ternatea L. methanolic leaf extract. Bioreduction of chloroauric acid (HAuCl₄) respectively, with the C. ternatea extract, this mixer incubated at 24 h and the mixer were characterized by UV–Vis spectroscopy, FTIR, XRD, TEM, EDS results were confirmed the presence of gold ion. Further, the synthesized bio-AuNPs were found to show highest antioxidant activity when compared with standard (vitamin C) using 2, 2′-azino-bi’s (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS+). Also, we evaluated the antimicrobial activity of bio-AuNPs against multidrug resistant Gram-negative bacteria and Gram-positive bacteria with maximum concentration (100 µL) was showing strong inhibitory action. In addition, the bio-AuNPs showed potential QSI activity with different concentration (25–100 µg/ml) against the AHL-mediated violacein production in C. violaceum (18–88 %) and biofilm formation (22–94 %), LasA protease (14–82 %), LasB elastase (15–81 %), pyocyanin (19–86 %) productions in P. aeruginosa and again it was confirmed by microscopy results and extracellular protein were also studied by SDS-PAGE method. The synthesized bio-AuNPs showed less hemolysis activity 3.75 %, compared with American Society for Testing and Materials range <5 %, it’s recommended that the bio-AuNPs were clearly much hemocompatible for drug delivery applications.