文摘
Based on the conclusions of the European Food Safety Authority (EFSA), the four polycyclic aromatic hydrocarbons (PAH4) chrysene (Chr), benzo(a)anthracene (BaA), benzo(a)pyrene (BaP), and benzo(b)fluoranthene (BbF) are new markers for the occurrence of PAHs in food. Vegetable oils are extremely easily contaminated by PAHs, due to the raw material pollution and the imperfections of processing techniques. Therefore, it is of great importance to establish an efficient method for the rapid determination of PAH4 in vegetable oils. In this research, second-derivative nonlinear variable-angle-matrix isopotential synchronous fluorescence spectroscopic approach has been proposed for the simultaneous and rapid determination of PAH4 in vegetable oils with ultrasonic-assisted extraction. This method is capable of resolving the spectra of these four PAHs well. The limits of detection (LODs) for Chr, BaA, BaP, and BbF were 13.0, 8.64, 0.16, and 7.68 μg kg−1, respectively. The recoveries in various oil samples for Chr, BaA, BaP, and BbF were 86.2 ± 15.3, 93.8 ± 11.7, 93.9 ± 12.1, and 94.3 ± 7.4 %, respectively, at the spiked levels of 10∼400 μg kg−1. The relative standard deviations (RSDs) achieved for Chr, BaA, BaP, and BbF were 3.0, 8.4, 6.3, and 7.2 %, respectively. The established method is simple, economical, and reliable, as well as provides an attractive alternative for the simultaneous and rapid determination of PAH4 in vegetable oils.