Cloning, overexpression and characterization of a new oligoalginate lyase from a marine bacterium, Shewanella sp.
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- 作者：Linna Wang ; Shangyong Li ; Wengong Yu ; Qianhong Gong
- 关键词：Biofuel ; Cloning ; Monomeric sugar acid ; Oligoalginate lyase OalS17 ; Shewanella sp. Kz7
- 刊名：Biotechnology Letters
- 出版年：2015
- 出版时间：March 2015
- 年：2015
- 卷：37
- 期：3
- 页码：665-671
- 全文大小：911 KB
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16. Wang, Y, Guo, EW, Yu, WG (2013) Purification and characterization of a new alginate lyase from a marine bacterium Vibrio sp. Biotechnol Lett 35: pp. 703-708- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Biotechnology
Applied Microbiology
Biochemistry- 出版者：Springer Netherlands
- ISSN：1573-6776
文摘
Purpose of work Is to report an oligoalginate lyase with high enzymatic activity and high-level expression. Using site-finding PCR and degenerate PCR, a gene (designated oalS17) encoding a new oligoalginate lyase was cloned from Shewanella sp. Kz7 and expressed in Escherichia coli. The gene consisted of 2,292?bp with deduced amino acid size of 763 including a putative signal peptide of 44 amino acid residues belonging to polysaccharide lyase (PL) family 17. The recombinant protein was most active at 50?°C and pH 6.2 in 50?mM phosphate buffer. It degraded alginate more efficiently than polyM and polyG block into a monomeric sugar acid, with a specific activity of 32?U?mg? toward alginate, 24?U?mg? toward polyM and 5?U?mg? toward polyG. With the high-level expression and high enzymatic activity, the recombinant oligoalginate lyase OalS17 could be a potential enzyme for further research on alginate saccharification and biofuels production.