Rapid isolation and sequence analysis of the beta-tubulin gene from Porphyra yezoensis (Rhodophyta)

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• 作者：Qianhong Gong (1)
  Feng Han (1)
  Jixun Dai (1)
  Hongquan Liu (1)
  Huashi Guan (1)
  Wengong Yu (1)
  
• 关键词：beta ; tubulin ; intronless ; inverse PCR ; Porphyra yezoensis ; Rhodophyta ; sequence analysis
• 刊名：Journal of Applied Phycology
• 出版年：2005
• 出版时间：January 2005
• 年：2005
• 卷：17
• 期：1
• 页码：1-5
• 全文大小：1606KB
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• 作者单位：Qianhong Gong (1)
  Feng Han (1)
  Jixun Dai (1)
  Hongquan Liu (1)
  Huashi Guan (1)
  Wengong Yu (1)
  
  1. Marine Drug and Food Institute, Ocean University of China, 5 Yushan Road, Qingdao, 266003, People’s Republic of China
  

文摘

Beta-tubulin, one of the cytoskeletal proteins, has been highly conserved throughout the evolution of eukaryotes. Degenerate PCR and inverse PCR (iPCR) were used to isolate the full-length beta-tubulin gene and its 5-and 3-flanking regions (2799bp) from the marine red alga Porphyra yezoensis. This gene, designated as TubB1, is devoid of introns. The canonical cis-acting elements such as TATA box, CAAT box and polyA signal AAUAAA are not found in flanking sequences, but another putative polyA signal CAYTG is found downstream of the stop codon. Comparison of the deduced 458 amino acid sequences shows higher similarity to the Protoflorideophycidae Cyanidioschyzon merolae (82%) than to the red alga Chondrus crispus (79%). Codon bias indicates strong expression of TubB1. Phylogenetic analysis suggests that the beta-tubulin of P. yezoensis and C. merola go together with fungi and not with green plants. These nucleotide sequence data have been deposited in the DDB/EMBL/Genbank databases under the accession number AY221630.