A novel Halomonas ventosae-specific virulent halovirus isolated from the Qiaohou salt mine in Yunnan, Southwest China
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- 作者：Chao-Qun Fu ; Qin Zhao ; Zhi-Ying Li ; Yong-Xia Wang ; Shi-Ying Zhang…
- 关键词：Siphovirus ; Halovirus ; Halomonas ; Salt mine ; Halophile ; Isolation and characterization
- 刊名：Extremophiles
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：20
- 期：1
- 页码：101-110
- 全文大小：741 KB
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Qin Zhao (1)
Zhi-Ying Li (1)
Yong-Xia Wang (1)
Shi-Ying Zhang (2)
Yong-Hong Lai (1)
Wei Xiao (1)
Xiao-Long Cui (1)
1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People’s Republic of China
2. Yunnan Engineering Laboratory of Soil Fertility and Pollution Remediation, Yunnan Agricultural University, Kunming, Yunnan, 650201, People’s Republic of China - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Biotechnology
Ecology - 出版者：Springer Japan
- ISSN：1433-4909
文摘
Although Halomonas phages belonging to the families Myoviridae and Siphoviridae have been reported, no virulent Halomonas siphoviruses are known. In this study, a virulent bacteriophage, QHHSV-1, of the family Siphoviridae that specifically infects H. ventosae QH52-2 was isolated from the Qiaohou salt mine. Restriction analysis indicated that QHHSV-1 is a dsDNA virus with a genome size of 33.5–39.5 kb. Transmission electron microscopy showed that QHHSV-1 is a typical representative of the Siphoviridae, with an icosahedral head (47 nm in diameter) and a non-contractile tail (75 nm in length). We also assessed the adsorption rate of QHHSV-1 for the host bacterium and found significant inhibition after the addition of 10 mM CaCl2. Based on a one-step growth curve, we determined a latent period of 30 min and a burst size of 73 PFU/infected cell. At the optimal pH of 8.0, 25.9 and 15.2 % of the phages survived after a 60-min incubation at 50 and 60 °C, respectively. Phage replication was possible at a wide range of salt concentrations, from 2.0 to 20 % (w/v), with an optimum concentration of 5 %. The survival of QHHSV-1 at different salt concentrations decreased with time and 25 % survival after 25 days at 30 % salt concentration. Keywords Siphovirus Halovirus Halomonas Salt mine Halophile Isolation and characterization