Down-regulation of Dicer in hepatocellular carcinoma

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• 作者：Jin-Feng Wu (1) (2)
  Wei Shen (2)
  Nian-Zhou Liu (3)
  Gui-Li Zeng (1) (2)
  Mei Yang (1) (2)
  Guo-Qing Zuo (2)
  Xiu-Ni Gan (4)
  Hong Ren (1)
  Kai-Fu Tang (1)
  
• 关键词：HCC ; Dicer ; Real ; time PCR
• 刊名：Medical Oncology
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：28
• 期：3
• 页码：804-809
• 全文大小：225KB
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• 作者单位：Jin-Feng Wu (1) (2)
  Wei Shen (2)
  Nian-Zhou Liu (3)
  Gui-Li Zeng (1) (2)
  Mei Yang (1) (2)
  Guo-Qing Zuo (2)
  Xiu-Ni Gan (4)
  Hong Ren (1)
  Kai-Fu Tang (1)
  
  1. Key Laboratory of Molecular Biology for Infectious Diseases of the State Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical University, 74# Linjiang Road, 400010, Chongqing, People鈥檚 Republic of China
  2. Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, 400010, Chongqing, People鈥檚 Republic of China
  3. Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, 400038, Chongqing, People鈥檚 Republic of China
  4. Department of Nursing, The Second Affiliated Hospital, Chongqing Medical University, 400010, Chongqing, People鈥檚 Republic of China
  

文摘

Dicer, the key enzyme in the RNAi pathway, is misregulated in tumor tissues. The altered expression of Dicer is associated with clinical characteristics in patients with cancer. Liver carcinoma and adjacent non-neoplastic tissues were obtained from 36 patients with hepatocellular carcinoma (HCC) undergoing surgery. Expressions of Dicer mRNA were evaluated using the Real-time reverse transcription-PCR in 36 liver carcinoma tissues and 36 adjacent histologically non-cancerous liver tissues. Dicer mRNA levels were evaluated in relation to age, sex, tumor number, tumor size, tumor stage, and distant metastasis. Dicer mRNA level was significantly lower in malignant tissues than in the corresponding non-neoplastic tissues in 34 of the 36 patients with HCC (94.4%). The Dicer expression level was not associated with clinical characteristics, including age, sex, tumor number, tumor size, tumor stage, or distant metastasis in HCC cases. These results demonstrate that Dicer is significantly down-regulated in HCC, suggesting that reduced expression of Dicer may play an important role during the process of hepatocarcinogenesis.