Genomic research for important pathogenic bacteria in China

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• 作者：RuiFu Yang (1)
  XiaoKui Guo (2)
  Jian Yang (3)
  YongQiang Jiang (1)
  Bo Pang (4)
  Chen Chen (5)
  YuFeng Yao (2)
  JinHong Qin (2)
  QingTian Li (2)
  
• 关键词：genome ; pathogenic bacteria ; sequencing ; application
• 刊名：Science China Life Sciences
• 出版年：2009
• 出版时间：January 2009
• 年：2009
• 卷：52
• 期：1
• 页码：50-63
• 全文大小：660KB
• 参考文献：1. Fleischmann R D, Adams M D, White O, et al. Whole-genome random sequencing and assembly of / Haemophilus influenzae Rd. Science, 1995, 269(5223): 496鈥?12
  2. Shendure J, Ji H. Next-generation DNA sequencing. Nat Biotechnol, 2008, 26(10): 1135鈥?145
  3. Schuster S C. Next-generation sequencing transforms today鈥檚 biology. Nat Methods, 2008, 5(1): 16鈥?8
  4. Holt K E, Parkhill J, Mazzoni C J, et al. High-throughput sequencing provides insights into genome variation and evolution in / Salmonella typhi. Nat Genet, 2008, 40(8): 987鈥?93
  5. Achtman M. Population structure of pathogenic bacteria revisited. Int J Med Microbiol, 2004, 294(2鈥?): 67鈥?3
  6. Achtman M, Morelli G, Zhu P, et al. Microevolution and history of the plague bacillus, / Yersinia pestis. Proc Natl Acad Sci USA, 2004, 101(51): 17837鈥?7842
  7. Achtman M. Evolution, population structure, and phylogeography of genetically monomorphic bacterial pathogens. Annu Rev Microbiol, 2008, 62: 53鈥?0
  8. Turner K M, Feil E J. The secret life of the multilocus sequence type. Int J Antimicrob Agents, 2007, 29(2): 129鈥?35
  9. Viratyosin W, Ingsriswang S, Pacharawongsakda E, et al. Genome- wide subcellular localization of putative outer membrane and extracellular proteins in / Leptospira interrogans serovar Lai genome using bioinformatics approaches. BMC Genomics, 2008, 9: 181
  10. Cui Y, Li Y, Gorge O, et al. Insight into microevolution of / Yersinia pestis by clustered regularly interspaced short palindromic repeats. PLoS ONE, 2008, 3(7): e2652
  11. Vergnaud G, Li Y, Gorge O, et al. Analysis of the three / Yersinia pestis CRISPR loci provides new tools for phylogenetic studies and possibly for the investigation of ancient DNA. Adv Exp Med Biol, 2007, 603: 327鈥?38
  12. Feil E J, Maiden M C, Achtman M, et al. The relative contributions of recombination and mutation to the divergence of clones of / Neisseria meningitidis. Mol Biol Evol, 1999, 16(11): 1496鈥?502
  13. Ren S X, Fu G, Jiang X G, et al. Unique physiological and pathogenic features of / Leptospira interrogans revealed by whole-genome sequencing. Nature, 2003, 422(6934): 888鈥?93
  14. Li Y, Dai E, Cui Y, et al. Different region analysis for genotyping / Yersinia pestis isolates from China. PLoS ONE, 2008, 3(5): e2166
  15. Adair D M, Worsham P L, Hill K K, et al. Diversity in a variable-number tandem repeat from / Yersinia pestis. J Clin Microbiol, 2000, 38(4): 1516鈥?519
  16. Klevytska A M, Price L B, Schupp J M, et al. Identification and characterization of variable-number tandem repeats in the / Yersinia pestis genome. J Clin Microbiol, 2001, 39(9): 3179鈥?185
  17. Pourcel C, Andre-Mazeaud F, Neubauer H, et al. Tandem repeats analysis for the high resolution phylogenetic analysis of / Yersinia pestis. BMC Microbiol, 2004, 4: 22
  18. Pourcel C, Salvignol G, Vergnaud G. CRISPR elements in / Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies. Microbiology, 2005, 151(Pt 3): 653鈥?63
  19. Guiyoule A, Grimont F, Iteman I, et al. Plague pandemics investigated by ribotyping of / Yersinia pestis strains. J Clin Microbiol, 1994, 32(3): 634鈥?41
  20. Guiyoule A, Rasoamanana B, Buchrieser C, et al. Recent emergence of new variants of / Yersinia pestis in Madagascar. J Clin Microbiol, 1997, 35(11): 2826鈥?833
  21. Huang F, Yu D, Hai R, et al. Study on the application of random amplified polymorphic DNA in / Yersinia pestis genotyping (in China). Chinese Journal of Epidemiology, 2000, 21(6): 424鈥?26
  22. Kumar S, Wang L, Fan J, et al. Detection of 11 common viral and bacterial pathogens causing community-acquired pneumonia or sepsis in asymptomatic patients by using a multiplex reverse transcription- PCR assay with manual (enzyme hybridization) or automated (electronic microarray) detection. J Clin Microbiol, 2008, 46(9): 3063鈥?072
  23. Leclercq A J, Torrea G, Chenal-Francisque V, et al. 3 IS-RFLP: a powerful tool for geographical clustering of global isolates of / Yersinia pestis. Adv Exp Med Biol, 2007, 603 322鈥?26
  24. Dorrell N, Hinchliffe S J, and Wren B W. Comparative phylogenomics of pathogenic bacteria by microarray analysis. Curr Opin Microbiol, 2005, 8(5): 620鈥?26
  25. Chen C, Tang J, Dong W, et al. A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates. PLoS ONE, 2007, 2(3): e315
  26. Wang J, Wang W, Li R, et al. The diploid genome sequence of an Asian individual. Nature, 2008, 456(7218): 60鈥?5
  27. Chain P S, Hu P, Malfatti S A, et al. Complete genome sequence of / Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen. J Bacteriol, 2006, 188(12): 4453鈥?463
  28. Parkhill J, Wren B W, Thomson N R, et al. Genome sequence of / Yersinia pestis, the causative agent of plague. Nature, 2001, 413(6855): 523鈥?27
  29. Read T D, Peterson S N, Tourasse N, et al. The genome sequence of / Bacillus anthracis Ames and comparison to closely related bacteria. Nature, 2003, 423(6935): 81鈥?6
  30. Enserink M, Malakoff D. / Bioterrorism. Congress weighs select agent update. Science, 2001, 294(5546): 1438
  31. Keim P. MICROBIAL FORENSICS: a scientific assessment. A Report from American Academy of Microbiology, 2003, http://www.asmusa.org
  32. Read T D, Salzberg S L, Pop M, et al. Comparative genome sequencing for discovery of novel polymorphisms in / Bacillus anthracis. Science, 2002, 296(5575): 2028鈥?033
  33. Gerner-Smidt P, Hise K, Kincaid J, et al. PulseNet USA: a five-year update. Foodborne Pathog Dis, 2006, 3(1): 9鈥?9
  34. Jackson C R, Fedorka-Cray P J, Wineland N, et al. Introduction to United States Department of Agriculture VetNet: status of Salmonella and Campylobacter databases from 2004 through 2005. Foodborne Pathog Dis, 2007, 4(2): 241鈥?48
  35. Garcia-Yoldi D, Le Fleche P, Marin C M, et al. Assessment of genetic stability of / Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis. Vaccine, 2007, 25(15): 2858鈥?862
  36. Aanensen D M, Spratt B G. The multilocus sequence typing network: mlst.net. Nucleic Acids Res, 2005, 33 (Web Server issue): W728鈥揥733
  37. Brudey K, Driscoll J R, Rigouts L, et al. / Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol, 2006, 6: 23
  38. Ahmed N, Majeed A A, Ahmed I, et al. genoBASE pylori: a genotype search tool and database of the human gastric pathogen / Helicobacter pylori. Infect Genet Evol, 2007, 7(4): 463鈥?68
  39. McEwen S A, Wilson T M, Ashford D A, et al. Microbial forensics for natural and intentional incidents of infectious disease involving animals. Rev Sci Tech, 2006, 25(1): 329鈥?39
  40. Vogler A J, Driebe E M, Lee J, et al. Assays for the rapid and specific identification of North American / Yersinia pestis and the common laboratory strain CO92. Biotechniques, 2008, 44(2): 201, 203鈥?04, 207
  41. Touchman J W, Wagner D M, Hao J, et al. A North American / Yersinia pestis draft genome sequence: SNPs and phylogenetic analysis. PLoS ONE, 2007, 2(2): e220
  42. Zhou D, Han Y, Song Y, et al. DNA microarray analysis of genome dynamics in / Yersinia pestis: insights into bacterial genome microevolution and niche adaptation. J Bacteriol, 2004, 186(15): 5138鈥?146
  43. Ciammaruconi A, Grassi S, De Santis R, et al. Fieldable genotyping of / Bacillus anthracis and / Yersinia pestis based on 25-loci Multi Locus VNTR Analysis. BMC Microbiol, 2008, 8: 21
  44. Vijaya Satya R, Zavaljevski N, Kumar K, et al. A high-throughput pipeline for designing microarray-based pathogen diagnostic assays. BMC Bioinformatics, 2008, 9: 185
  45. Ben R J, Kung S, Chang F Y, et al. Rapid diagnosis of bacterial meningitis using a microarray. J Formos Med Assoc, 2008, 107(6): 448鈥?53
  46. Grimm V, Ezaki S, Susa M, et al. Use of DNA microarrays for rapid genotyping of TEM beta-lactamases that confer resistance. J Clin Microbiol, 2004, 42(8): 3766鈥?774
  47. Sachse K, Laroucau K, Hotzel H, et al. Genotyping of / Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes. BMC Microbiol, 2008, 8: 63
  48. Monecke S, Jatzwauk L, Weber S, et al. DNA microarray-based genotyping of methicillin-resistant / Staphylococcus aureus strains from Eastern Saxony. Clin Microbiol Infect, 2008, 14(6): 534鈥?45
  49. Ballmer K, Korczak B M, Kuhnert P, et al. Fast DNA serotyping of / Escherichia coli by use of an oligonucleotide microarray. J Clin Microbiol, 2007, 45(2): 370鈥?79
  50. Stabler R A, Dawson L F, Oyston P C, et al. Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux. BMC Microbiol, 2008, 8(1): 177
  51. Rehrauer H, Schonmann S, Eberl L, et al. PhyloDetect: a likelihood- based strategy for detecting microorganisms with diagnostic microarrays. Bioinformatics, 2008, 24(16): i83鈥搃89
  52. Tembe W, Zavaljevski N, Bode E, et al. Oligonucleotide fingerprint identification for microarray-based pathogen diagnostic assays. Bioinformatics, 2007, 23(1): 5鈥?3
  53. Kostic T, Weilharter A, Rubino S, et al. A microbial diagnostic microarray technique for the sensitive detection and identification of pathogenic bacteria in a background of nonpathogens. Anal Biochem, 2007, 360(2): 244鈥?54
  54. Capecchi B, Serruto D, Adu-Bobie J, et al. The genome revolution in vaccine research. Curr Issues Mol Biol, 2004, 6(1): 17鈥?7
  55. Masignani V, Rappuoli R, and Pizza M. Reverse vaccinology: a genome-based approach for vaccine development. Expert Opin Biol Ther, 2002, 2(8): 895鈥?05
  56. Holst J. Strategies for development of universal vaccines against meningococcal serogroup B disease: the most promising options and the challenges evaluating them. Hum Vaccin, 2007, 3(6): 290鈥?94
  57. Koizumi N, and Watanabe H. Leptospirosis vaccines: past, present, and future. J Postgrad Med, 2005, 51(3): 210鈥?14
  58. Li B, Zhou D, Wang Z, et al. Antibody profiling in plague patients by protein microarray. Microbes Infect, 2008, 10(1): 45鈥?1
  59. Chen Z, Li B, Zhang J, et al. Quorum sensing affects virulence- associated proteins F1, LcrV, KatY and pH6 etc. of / Yersinia pestis as revealed by protein microarray-based antibody profiling. Microbes Infect, 2006, 8(9鈥?0): 2501鈥?508
  60. Li B, Jiang L, Song Q, et al. Protein microarray for profiling antibody responses to / Yersinia pestis live vaccine. Infect Immun, 2005, 73(6): 3734鈥?739
  61. Henics T, Winkler B, Pfeifer U, et al. Small-fragment genomic libraries for the display of putative epitopes from clinically significant pathogens. Biotechniques, 2003, 35(1): 196鈥?02, 204, 206 passim
  62. Khan A S, Mujer C V, Alefantis T G, et al. Proteomics and bioinformatics strategies to design countermeasures against infectious threat agents. J Chem Inf Model, 2006, 46(1): 111鈥?15
  63. Dietrich J, Weldingh K, Andersen P. Prospects for a novel vaccine against tuberculosis. Vet Microbiol, 2006, 112(2鈥?): 163鈥?69
  64. Telford J L. Bacterial genome variability and its impact on vaccine design. Cell Host Microbe, 2008, 3(6): 408鈥?16
  65. Jagusztyn-Krynicka E K, Wyszynska A. The decline of antibiotic era-new approaches for antibacterial drug discovery. Pol J Microbiol, 2008, 57(2): 91鈥?8
  66. Szekely R, Waczek F, Szabadkai I, et al. A novel drug discovery concept for tuberculosis: inhibition of bacterial and host cell signalling. Immunol Lett, 2008, 116(2): 225鈥?31
  67. Suga H, Smith K M. Molecular mechanisms of bacterial quorum sensing as a new drug target. Curr Opin Chem Biol, 2003, 7(5): 586鈥?91.
  68. Zheng H, Lu L, Wang B, et al. Genetic basis of virulence attenuation revealed by comparative genomic analysis of / Mycobacterium tuberculosis strain H37Ra versus H37Rv. PLoS ONE, 2008, 3(6): e2375
  69. Pucci M J. Use of genomics to select antibacterial targets. Biochem Pharmacol, 2006, 71(7): 1066鈥?072
  70. da Silveira N J, Bonalumi C E, Uchoa H B, et al. DBMODELING: a database applied to the study of protein targets from genome projects. Cell Biochem Biophys, 2006, 44(3): 366鈥?74
  71. Singh N K, Selvam S M, Chakravarthy P. T-iDT: tool for identification of drug target in bacteria and validation by / Mycobacterium tuberculosis. In Silico Biol, 2006, 6(6): 485鈥?93
  72. Purohit H J, Cheema S, Lal S, et al. In search of drug targets for / Mycobacterium tuberculosis. Infect Disord Drug Targets, 2007, 7(3): 245鈥?50
  73. McLean K J, Munro A W. Structural biology and biochemistry of cytochrome P450 systems in / Mycobacterium tuberculosis. Drug Metab Rev, 2008, 40(3): 427鈥?46
  74. Holton S J, Weiss M S, Tucker P A, et al. Structure-based approaches to drug discovery against tuberculosis. Curr Protein Pept Sci, 2007, 8(4): 365鈥?75
  75. Li M, Wang B, Zhang M, et al. Symbiotic gut microbes modulate human metabolic phenotypes. Proc Natl Acad Sci U S A, 2008, 105(6): 2117鈥?122
  76. Frank D N, Pace N R. Gastrointestinal microbiology enters the metagenomics era. Curr Opin Gastroenterol, 2008, 24(1): 4鈥?0 CrossRef
  77. Ley R E, Turnbaugh P J, Klein S, et al. Microbial ecology: human gut microbes associated with obesity. Nature, 2006, 444(7122): 1022鈥?023
  78. Marchesi J, Shanahan F. The normal intestinal microbiota. Curr Opin Infect Dis, 2007, 20(5): 508鈥?13
  79. von Bubnoff A. Next-generation sequencing: the race is on. Cell, 2008, 132(5): 721鈥?23
  80. Korlach J, Bibillo A, Wegener J, et al. Long, processive enzymatic DNA synthesis using 100% dye-labeled terminal phosphate-linked nucleotides. Nucleosides Nucleotides Nucleic Acids, 2008, 27(9): 1072鈥?083
  81. Kahvejian A, Quackenbush J, Thompson J F. What would you do if you could sequence everything? Nat Biotechnol, 2008, 26(10): 1125鈥?133
  82. Jin Q, Yuan Z, Xu J, et al. Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of / Escherichia coli K12 and O157. Nucleic Acids Res, 2002, 30(20): 4432鈥?441
  83. Nie H, Yang F, Zhang X, et al. Complete genome sequence of / Shigella flexneri 5b and comparison with / Shigella flexneri 2a. BMC Genomics, 2006, 7: 173
  84. Yang F, Yang J, Zhang X, et al. Genome dynamics and diversity of / Shigella species, the etiologic agents of bacillary dysentery. Nucleic Acids Res, 2005, 33(19): 6445鈥?458
  85. Yang J, Chen L, Yu J, et al. ShiBASE: an integrated database for comparative genomics of / Shigella. Nucleic Acids Res, 2006, 34 (Database issue): D398鈥?01
  86. Vinetz J M. Leptospirosis. Curr Opin Infect Dis, 2001, 14(5): 527鈥?38
  87. Guerreiro H, Croda J, Flannery B, et al. Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans. Infect Immun, 2001, 69(8): 4958鈥?968
  88. Hindson B J, Makarewicz A J, Setlur U S, et al. APDS: the autonomous pathogen detection system. Biosens Bioelectron, 2005, 20(10): 1925鈥?931
  89. Bulach D M, Zuerner R L, Wilson P, et al. Genome reduction in / Leptospira borgpetersenii reflects limited transmission potential. Proc Natl Acad Sci U S A, 2006, 103(39): 14560鈥?4565
  90. Nascimento A L, Verjovski-Almeida S, Van Sluys M A, et al. Genome features of / Leptospira interrogans serovar / Copenhageni. Braz J Med Biol Res, 2004, 37(4): 459鈥?77
  91. Picardeau M, Bulach D M, Bouchier C, et al. Genome sequence of the saprophyte / Leptospira biflexa provides insights into the evolution of / Leptospira and the pathogenesis of leptospirosis. PLoS ONE, 2008, 3(2): e1607
  92. Qin J H, Sheng Y Y, Zhang Z M, et al. Genome-wide transcriptional analysis of temperature shift in / L. interrogans serovar lai strain 56601. BMC Microbiol, 2006, 6: 51
  93. He P, Sheng Y Y, Shi Y Z, et al. Genetic diversity among major endemic strains of / Leptospira interrogans in China. BMC Genomics, 2007, 8: 204
  94. Yang H L, Zhu Y Z, Qin J H, et al. In silico and microarray-based genomic approaches to identifying potential vaccine candidates against / Leptospira interrogans. BMC Genomics, 2006, 7: 293
  95. Cullen P A, Cordwell S J, Bulach D M, et al. Global analysis of outer membrane proteins from / Leptospira interrogans serovar Lai. Infect Immun, 2002, 70(5): 2311鈥?318
  96. Cullen P A, Xu X, Matsunaga J, et al. Surfaceome of / Leptospira spp. Infect Immun, 2005, 73(8): 4853鈥?863
  97. Nally J E, Whitelegge J P, Aguilera R, et al. Purification and proteomic analysis of outer membrane vesicles from a clinical isolate of / Leptospira interrogans serovar / Copenhageni. Proteomics, 2005, 5(1): 144鈥?52
  98. Amutha R, Chaudhuri P, Garg A P, et al. Immunoreactive outer membrane proteins of Leptospira interrogans serovar Canicola strain Hond Utrecht IV. Indian J Med Res, 2006, 124(5): 569鈥?74
  99. Nally J E, Whitelegge J P, Bassilian S, et al. Characterization of the outer membrane proteome of / Leptospira interrogans expressed during acute lethal infection. Infect Immun, 2007, 75(2): 766鈥?73
  100. Sakolvaree Y, Maneewatch S, Jiemsup S, et al. Proteome and immunome of pathogenic / Leptospira spp. revealed by 2DE and 2DE-immunoblotting with immune serum. Asian Pac J Allergy Immunol, 2007, 25(1): 53鈥?3
  101. Cole S T, Brosch R, Parkhill J, et al. Deciphering the biology of / Mycobacterium tuberculosis from the complete genome sequence. Nature, 1998, 393(6685): 537鈥?44
  102. Chesne-Seck M L, Barilone N, Boudou F, et al. A point mutation in the two-component regulator PhoP-PhoR accounts for the absence of polyketide-derived acyltrehaloses but not that of phthiocerol dimycocerosates in / Mycobacterium tuberculosis H37Ra. J Bacteriol, 2008, 190(4): 1329鈥?334
  103. Frigui W, Bottai D, Majlessi L, et al. Control of / M. tuberculosis ESAT-6 secretion and specific T cell recognition by PhoP. PLoS Pathog, 2008, 4(2): e33
  104. Anisimov A P, Lindler L E, Pier G B. Intraspecific diversity of / Yersinia pestis. Clin Microbiol Rev, 2004, 17(2): 434鈥?64
  105. Perry R D, Fetherston J D. / Yersinia pestis鈥攅tiologic agent of plague. Clin Microbiol Rev, 1997, 10(1): 35鈥?6
  106. Perry R D, Straley S C, Fetherston J D, et al. DNA sequencing and analysis of the low-Ca²⁺-response plasmid pCD1 of / Yersinia pestis KIM5. Infect Immun, 1998, 66(10): 4611鈥?623
  107. Deng W, Burland V, Plunkett G 3rd, et al. Genome sequence of / Yersinia pestis KIM. J Bacteriol, 2002, 184(16): 4601鈥?611
  108. Song Y, Tong Z, Wang J, et al. Complete genome sequence of / Yersinia pestis strain 91001, an isolate avirulent to humans. DNA Res, 2004, 11(3): 179鈥?97
  109. Zhou D, Tong Z, Song Y, et al. Genetics of metabolic variations between / Yersinia pestis biovars and the proposal of a new biovar, microtus. J Bacteriol, 2004, 186(15): 5147鈥?152
  110. Zhou D, Han Y, Song Y, et al. Comparative and evolutionary genomics of / Yersinia pestis. Microbes Infect, 2004, 6(13): 1226鈥?234
  111. Dai E, Tong Z, Wang X, et al. Identification of different regions among strains of / Yersinia pestis by suppression subtractive hybridization. Res Microbiol, 2005, 156(7): 785鈥?89
  112. Pang B, Yan M, Cui Z, et al. Genetic diversity of toxigenic and nontoxigenic / Vibrio cholerae serogroups O1 and O139 revealed by array- based comparative genomic hybridization. J Bacteriol, 2007, 189(13): 4837鈥?849
  113. Sun J C, Cao X, Liu J, et al. Prediction and systematic study of protein- protein interaction networks of Leptospira interrogans. Chin Sci Bull, 2006, 51(11): 1296鈥?305
  114. Geng H, Zhu L, Yuan Y, et al. Identification and characterization of novel immunogenic proteins of / Streptococcus suis serotype 2. J Proteome Res, 2008, 7(9): 4132鈥?142
  115. Li M, Wang C, Feng Y, et al. SalK/SalR, a two-component signal transduction system, is essential for full virulence of highly invasive / Streptococcus suis serotype 2. PLoS ONE, 2008, 3(5): e2080
  116. Zhang Y Q, Ren S X, Li H L, et al. Genome-based analysis of virulence genes in a non-biofilm-forming / Staphylococcus epidermidis strain (ATCC 12228). Mol Microbiol, 2003, 49(6): 1577鈥?593
  117. Qin Z, Yang X, Yang L, et al. Formation and properties of in vitro biofilms of ica-negative / Staphylococcus epidermidis clinical isolates. J Med Microbiol, 2007, 56(Pt 1): 83鈥?3
  118. Li H, Xu L, Wang J, et al. Conversion of / Staphylococcus epidermidis strains from commensal to invasive by expression of the ica locus encoding production of biofilm exopolysaccharide. Infect Immun, 2005, 73(5): 3188鈥?191
  119. Wang C, Li M, Dong D, et al. Role of ClpP in biofilm formation and virulence of / Staphylococcus epidermidis. Microbes Infect, 2007, 9(11): 1376鈥?383
  120. Qin Z, Ou Y, Yang L, et al. Role of autolysin-mediated DNA release in biofilm formation of / Staphylococcus epidermidis. Microbiology, 2007, 153(Pt 7): 2083鈥?092
  121. Wu Y, Yu K, Xu B, et al. Potent and selective inhibitors of / Staphylococcus epidermidis tryptophanyl-tRNA synthetase. J Antimicrob Chemother, 2007, 60(3): 502鈥?09
  122. Qin Z, Lee B, Yang L, et al. Antimicrobial activities of YycG histidine kinase inhibitors against / Staphylococcus epidermidis biofilms. FEMS Microbiol Lett, 2007, 273(2): 149鈥?56
  123. Peng J, Yang L, Yang F, et al. Characterization of ST-4821 complex, a unique / Neisseria meningitidis clone. Genomics, 2008, 91(1): 78鈥?7
  124. Zhang X, Shao Z, Yang E, et al. Molecular characterization of serogroup C / Neisseria meningitidis isolated in China. J Med Microbiol, 2007, 56(Pt 9): 1224鈥?229
  125. Zhang X, Shao Z, Zhu Y, et al. Genetic characteristics of serogroup A meningococci circulating in China, 1956鈥?005. Clin Microbiol Infect, 2008, 14(6): 555鈥?61
  126. Yang L, Shao Z, Zhang X, et al. Genotypic characterization of / Neisseria meningitidis serogroup B strains circulating in China. J Infect, 2008, 56(3): 211鈥?18
  127. Yang J, Zhang X, Xu X, et al. Genotypic analysis of serogroups other than A, B or C of / Neisseria meningitidis in China. Scand J Infect Dis, 2007, 39(9): 819鈥?21
  128. Peng J, Zhang X, Yang E, et al. Characterization of serogroup C meningococci isolated from 14 provinces of China during 1966鈥?005 using comparative genomic hybridization. Sci China Ser. C Life Sci, 2007, 50(1): 1鈥?
  129. Peng J, Zhang X, Shao Z, et al. Characterization of a new Neisseria / meningitidis serogroup C clone from China. Scand J Infect Dis, 2008, 40(1): 63鈥?6
  
• 作者单位：RuiFu Yang (1)
  XiaoKui Guo (2)
  Jian Yang (3)
  YongQiang Jiang (1)
  Bo Pang (4)
  Chen Chen (5)
  YuFeng Yao (2)
  JinHong Qin (2)
  QingTian Li (2)
  
  1. Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China
  2. Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China
  3. State Key Laboratory for Molecular Virology and Genetic engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, 100176, China
  4. State Key Laboratory for Infectious Disease Prevention and Control, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China
  5. Beijing Genomics Institute at Shenzhen, Shenzhen, 518083, China
  

文摘

Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of point. We can thus closely look at the way in which a pathogen is evolved, and these data has been applied to molecular epidemiology and microbial forensics, and screening of novel diagnostic, vaccine and drug targets. The newly developed high-throughput low-cost sequencing technologies, such as 454, Solexa and SOLiD, will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously, such as the metagenomics of human gastric-intestinal tract, for better and comprehensive understanding of human health and disease.