Validated LC-ESI-MS/MS Method for the Quantitation of Neopanaxadiol: a Novel Neuroprotective Agent from Panax ginseng and Its Application to a Pharmacokinetic Study in Rat Plasma

详细信息    查看全文

• 作者：Cong Geng (1)
  Jihua Liu (1)
  Qingxiang Guan (1)
  Ying Zhang (1)
  Qin Meng (1)
  Deya Sun (1)
  Yuequan Sun (2)
  Jianwei Wang (2)
  Yihan Zhou (2)
  Jianyuan Yin (1)
  
• 关键词：UPLC ; ESI ; MS/MS ; Neopanaxadiol ; Ginsenoside ; Pharmacokinetics ; Rat plasma
• 刊名：Chromatographia
• 出版年：2013
• 出版时间：10 - May 2013
• 年：2013
• 卷：76
• 期：9
• 页码：509-514
• 全文大小：291KB
• 参考文献：1. Tao LN, Meng Q, Yin JY, Xing R, Guo HR (2009) Chin Chem Lett 20:687-89 CrossRef
  2. Chen LM, Lin ZY, Zhu YQ, Lin N, Zhang J, Pan XD, Chen XC (2012) Eur J Pharmacol 675:15-1 CrossRef
  3. Peng L, Sun S, Xie LH, Wicks SM, Xie JT (2012) Cardiovasc Ther 30:e183–e188 CrossRef
  4. Lee ES, Choi JS, Kim MS, You HJ, Ji GE, Kang YH (2011) Chem Biol Interact 194:13-2 CrossRef
  5. Cheng Y, Shen LH, Zhang JT (2005) Acta Pharmacol Sin 26:143-49 CrossRef
  6. Zhang C, Liu LJ, Yu Y, Chen B, Tang CW, Li X (2012) Mol Med Report 5:1295-298
  7. Wang W, Wang H, Rayburn ER, Zhao Y, Hill DL, Zhang R (2008) Br J Cancer 98:792-02 CrossRef
  8. Lee J, Lee E, Kim D, Lee J, Yoo J, Koh B (2009) J Ethnopharmacol 122:143-48 CrossRef
  9. Qian TX, Jiang ZH, Cai ZW (2006) Anal Biochem 352:87-6 CrossRef
  10. Chen GT, Yang M, Song Y, Lu ZQ, Zhang JQ, Huang HL, Guan SH, Wu LJ, Guo DA (2008) Biomed Chromatogr 22:779-85 CrossRef
  11. Wang XY, Zhao T, Gao XF, Dan M, Zhou MM, Jia W (2007) Anal Chim Acta 594:265-73 CrossRef
  12. Zhang XR, Zhang D, Xu JH, Gu JK, Zhao YQ (2007) J Chromatogr B 858:65-0 CrossRef
  13. Wang WY, Shao YF, Ma SS, Wu XM, Meng QG (2012) J Chromatogr B 887-88:19-4 CrossRef
  14. Liu L, Huang JQ, Hu X, Li KX, Sun CH (2011) J Chromatogr B 879:2011-017 CrossRef
  15. Chambers E, Wagrowski-Diehl DM, Lu ZL, Mazzeo JR (2007) J Chromatogr B 852:22-4 CrossRef
  16. Han MH, Chen J, Chen SL, Wang XT (2010) Anal Sci 26:749-53 CrossRef
  17. Joo KM, Lee JH, Jeon HY, Park CW, Hong DK, Jeong HJ, Lee SJ, Lee SY, Lim KM (2010) J Pharm Biomed Anal 51:278-83 CrossRef
  18. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Center for Veterinary Medicine (CVM), May, 2001
  
• 作者单位：Cong Geng (1)
  Jihua Liu (1)
  Qingxiang Guan (1)
  Ying Zhang (1)
  Qin Meng (1)
  Deya Sun (1)
  Yuequan Sun (2)
  Jianwei Wang (2)
  Yihan Zhou (2)
  Jianyuan Yin (1)
  
  1. Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, China
  2. National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, 130022, China
  
• ISSN：1612-1112

文摘

Neopanaxadiol (NPD), a new panaxadiol first obtained from the acid hydrolysate of the total ginsenosides of Panax ginseng C. A. Meyer (Araliaceae) in our laboratory, showed neuroprotective effect against glutamate-induced neurotoxicity in primary cultures of rat cortical cells (Tao et al. Chin Chem Lett 20:687-89, 2009). In this research, a sensitive and specifical method of ultra-performance liquid chromatography–electrospray mass spectrometry (UPLC-ESI-MS/MS) for the quantitative analysis of NPD in rat plasma was developed. The NPD and internal standard of panaxadiol were extracted from 200?μL of rat plasma samples with methanol, then the samples were injected in an UPLC (Waters BEH) with C18 column (50?mm?×?2.1?mm i.d., 1.7?μm) and eluted with the mobile phase consisting of methanol–water–formic acid (80:20:0.2, v/v/v). Excellent linearity was found between 5 and 160?ng?mL? with a limit of quantitation of 4?ng?mL?. Intra- and inter-day precision values (RSD) of QC samples were both below 7?%. This study was successfully utilized for the pharmacokinetic profiles of NPD in rats after oral administration and provided an experimental basis for the further pharmacology and clinical application of ginseng products.