Cloning and characterization of squalene synthase gene from Poria cocos and its up-regulation by methyl jasmonate
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- 作者：Jian-Rong Wang (1) (3)
Jun-Fang Lin (1) (2)
Li-Qiong Guo (1) (2)
Lin-Feng You (1) (2)
Xian-Lu Zeng (1) (2)
Jia-Ming Wen (1) - 关键词：Poria cocos ; Squalene synthase ; Triterpenoids ; Squalene ; Methyl jasmonate
- 刊名：World Journal of Microbiology and Biotechnology
- 出版年：2014
- 出版时间：February 2014
- 年：2014
- 卷：30
- 期：2
- 页码：613-620
- 全文大小：771 KB
- 作者单位：Jian-Rong Wang (1) (3)
Jun-Fang Lin (1) (2)
Li-Qiong Guo (1) (2)
Lin-Feng You (1) (2)
Xian-Lu Zeng (1) (2)
Jia-Ming Wen (1)
1. Department of Bioengineering, College of Food Science, South China Agricultural University, 482 Wu-Shan Road, Tian-He District, Guangzhou, 510642, Guangdong, China
3. Guangdong VTR Bio-Tech Co., Ltd., Zhuhai, China
2. Institute of Biomass Energy Research, South China Agricultural University, Guangzhou, 510642, Guangdong, China - ISSN：1573-0972
文摘
Squalene synthase (SQS) catalyzes the condensation of two molecules of farnesyl diphosphate to give presqualene diphosphate and the subsequent rearrangement to form squalene. The gene encoding squalene synthase was cloned from Poria cocos by degenerate PCR and inverse PCR. The open reading frame of the gene is 1,497?bp, which encodes 499 amino acid residues. A phylogenetic analysis revealed that P. cocos SQS belonged to the fungus group, and was more closely related to the SQS of Ganoderma lucidum than other fungi. The treatment of P. cocos with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of P. cocos sqs gene and the content of squalene in P. cocos. The transcriptional level of sqs gene was approximately fourfold higher than the control sample and the squalene content reached 128.62?μg/g, when the concentration of MeJA was 300?μM after 72?h induction.