Core 2 Mucin-Type O-Glycan Is Related to EPEC and EHEC O157:H7 Adherence to Human Colon Carcinoma HT-29 Epithelial Cells

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• 作者：Jun Ye ; Lili Song ; Yun Liu ; Qiong Pan ; Xiaoli Zhong…
• 关键词：Mucin ; O ; Glycan ; Adherence ; Enteropathogenic E. coli ; Enterohemorrhagic E. coli O157 ; H7
• 刊名：Digestive Diseases and Sciences
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：60
• 期：7
• 页码：1977-1990
• 全文大小：7,036 KB
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  25.Ya
• 作者单位：Jun Ye (1)
  Lili Song (1)
  Yun Liu (1)
  Qiong Pan (1)
  Xiaoli Zhong (1)
  Shanshan Li (1)
  Yangyang Shang (1)
  Yin Tian (1)
  Yonghong He (1)
  Lei Chen (1)
  Wensheng Chen (1)
  Zhihong Peng (1)
  Rongquan Wang (1)
  
  1. Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People’s Republic of China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Gastroenterology
  Hepatology
  Oncology
  Transplant Surgery
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-2568

文摘

Background and Aim The roles of host glycosylation in interactions with EPEC and EHEC O157:H7 are largely unclear; this study examined whether O-glycans are involved in EPEC and EHEC O157:H7 adherence to HT-29 cells. Methods Bacterial adherence to the cultured cells was determined using the direct co-staining of adherent bacteria and host cells, the adherent bacteria plating, and/or the direct fluorescent observation of the adherent GFP-labeled bacteria. Results A comparison of the adherence of EPEC and EHEC O157:H7 to HT-29-Gal and HT-29 cells indicated that the differentiation of HT-29 cells led to a reduction in the adherence of EPEC and EHEC O157:H7. EPEC and EHEC O157:H7 adhesion decreased after the abrogation of O-glycan biosynthesis mediated by benzyl-α-GalNAc treatment. Core 2 O-glycan-deficient HT-29 cells induced by C2GnT2 knockdown had a significant reduction in EPEC and EHEC O157:H7 adhesion in C2GnT2-sh2/HT-29 cells compared with HT-29 and shRNA-Ctr/HT-29 cells. MUC2 expression in benzyl-α-GalNAc-treated HT-29 cells was significantly reduced but unchanged in C2GnT2-deficient HT-29 cells. EPEC or EHEC O157:H7 infection in C2GnT2-deficient HT-29 cells deteriorated the epithelial barrier function. The occludin expression in the shRNA-Ctr/HT-29 and C2GnT2-sh2/HT-29 cells after infection with EPEC or EHEC O157:H7 was pyknic and discontinuous at the cell surface compared with its continuous distribution of control cells. These data indicate that EPEC and EHEC O157:H7 adherence to HT-29 cells is related to mucin-type core 2 O-glycan. Conclusions This study provides the concepts toward the design of carbohydrate-dependent inhibition of EPEC and EHEC O157:H7 adhesion to human intestinal epithelial cells.