Lanthanum Induced Primary Neuronal Apoptosis Through Mitochondrial Dysfunction Modulated by Ca2+ and Bcl-2 Family

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• 作者：Jie Wu (1) (2) (3)
  Jinghua Yang (1) (3)
  Qiufang Liu (1) (3)
  Shengwen Wu (1) (3)
  Honglin Ma (2)
  Yuan Cai (1) (3)
  
• 关键词：Lanthanum ; Cortical neuron ; Apoptosis ; Mitochondria ; Bcl ; 2
• 刊名：Biological Trace Element Research
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：152
• 期：1
• 页码：125-134
• 全文大小：523KB
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• 作者单位：Jie Wu (1) (2) (3)
  Jinghua Yang (1) (3)
  Qiufang Liu (1) (3)
  Shengwen Wu (1) (3)
  Honglin Ma (2)
  Yuan Cai (1) (3)
  
  1. Department of Toxicology, School of Public Health, China Medical University, Shenyang, 110001, China
  2. Department of Occupational and Environmental Health, School of Public Health, Liaoning Medical University, Jinzhou, 121001, China
  3. Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, 110001, China
  

文摘

As a representative element of lanthanide, lanthanum has been widely used in various fields and eventually entered environment and accumulated in human body. Epidemiological and experimental evidences indicated that lanthanum has neurotoxicity; however, the detailed mechanism is still elusive. Here, we chose primary cerebral cortical neurons as model in vitro to investigate the mechanism underlying the toxic effects of lanthanum chloride (LaCl3). This study revealed the following findings: (1) LaCl3 treatment (0.01, 0.1, and 1.0?mM for 24?h) reduced the viability of cortical neurons and elevated apoptotic rate significantly in a dose-dependent manner. (2) LaCl3 triggered mitochondrial apoptotic pathway in cortical neurons, characterized with collapsed mitochondrial membrane potential, release of cytochrome c into cytosol, and increasing expression of activated caspase-3. (3) LaCl3 elevated intracellular Ca2+ concentration, promoted reactive oxygen species generation, and upregulated pro-apoptotic Bax, whereas it downregulated anti-apoptotic Bcl-2 expression and consequently altered Bax/Bcl-2 ratio, which ultimately lead to neuronal mitochondrial apoptosis. Our results demonstrated that toxicity of lanthanum in cortical neurons perhaps partly attributed to enhanced mitochondrial apoptosis due to mitochondrial dysfunction modulated by Ca2+ and Bcl-2 family.