Molecular characterization of heat–shock protein 90 gene and its expression in Gobiocypris rarus juveniles exposed to pentachlorophenol

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• 作者：Qiuping Liu ; Shuting Huang ; Chuan Deng ; Li Xiong…
• 关键词：Gobiocypris rarus ; HSP90 ; RACE ; Pentachlorophenol ; Expression
• 刊名：Fish Physiology and Biochemistry
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：41
• 期：5
• 页码：1279-1291
• 全文大小：2,689 KB
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• 作者单位：Qiuping Liu (1)
  Shuting Huang (1)
  Chuan Deng (1)
  Li Xiong (1)
  Xiang Gao (1)
  Yun Chen (1)
  Chunqing Niu (1)
  Yan Liu (1)
  
  1. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, 400715, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Animal Physiology
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Zoology
  
• 出版者：Springer Netherlands
• ISSN：1573-5168

文摘

Heat–shock protein 90 (HSP90) is an abundant and highly conserved molecular chaperone, and it fulfills a housekeeping function in contributing to the folding, maintenance of structural integrity, and proper regulation of a subset of cytosolic proteins. In this study, the full-length 2693-bp cDNA of HSP90 was cloned by rapid amplification of cDNA ends (RACE) technique from the liver of rare minnow (Gobiocypris rarus) for the first time, designated as GrHSP90. The complete coding sequence of GrHSP90 is 2181 bp in length, which encodes a polypeptide of 726 amino acids with a predicted molecular mass of 83.4 kDa and a theoretical isoelectric point of 4.90. Phylogenetic tree analysis indicated that deduced protein GrHSP90 had extensive sequence similarities to other fish HSP90s. Tissue distribution showed that GrHSP90 was constitutively expressed in a wide range of tissues including gill, blood, brain, fin, gonad, heart, intestine, kidney, liver, muscle, spleen, skin, and swim bladder. The highest expression was found in the gonad. Furthermore, significant increase in GrHSP90 mRNA in the liver was observed after exposure to pentachlorophenol ? μg/L (p < 0.05). Our results suggest that GrHSP90 is indeed an ortholog of the HSP90 family and may be act as a biomarker to assess the effect of environmental contaminant. Keywords Gobiocypris rarus HSP90 RACE Pentachlorophenol Expression