Transfer of degradative plasmids from a rhizobacterium to single and multiple mixed recipients

详细信息    查看全文

• 作者：Heping Wei (1)
  Yujing Wang (2)
  Chenhao Zhou (2)
  Qiuyue Jiang (2)
  Xiaoli He (2)
  Jun Chen (2)
  Ming Xiao (2)
  
• 关键词：Biodegradation ; Transfer frequency ; Remediation ; Rhizosphere
• 刊名：Annals of Microbiology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：64
• 期：4
• 页码：1869-1873
• 全文大小：378 KB
• 参考文献：1. Barac T, Taghavi S, Borremans B, Provoost A, Oeyen L, Colpaert JV, Vangronsveld J, van der Lelie D (2004) Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants. Nat Biotechnol 22:583-88 CrossRef
  2. Eltis LD, Bolin JT (1996) Evolutionary relationships among extradiol dioxygenases. J Bacteriol 178:5930-937
  3. Ikuma K, Holzem RM, Gunsch CK (2012) Impacts of organic carbon availability and recipient bacteria characteristics on the potential for TOL plasmid genetic bioaugmentation in soil slurries. Chemosphere 89:158-63 CrossRef
  4. Li H, Li H, Bai Y, Wang J, Nie M, Li B, Xiao M (2011) The use of Pseudomonas fluorescens P13 to control sclerotinia stem rot ( / Sclerotinia sclerotiorum) of oilseed rape. J Microbiol 49:884-89 CrossRef
  5. Ma Y, Prasad MN, Rajkumar M, Freitas H (2011) Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils. Biotechnol Adv 29:248-58 CrossRef
  6. Pelz O, Tesar M, Wittich RM, Moore ERB, Timmis KN, Abraham WR (1999) Towards elucidation of microbial community metabolic pathways; unraveling the network of carbon sharing in a pollutant-degrading bacterial consortium by immunocapture and isotopic ratio mass spectrometry. Environ Microbiol 1:167-74 CrossRef
  7. Rahman KS, Rahman T, Lakshmanaperumalsamy P, Banat IM (2002) Occurrence of crude oil degrading bacteria in gasoline and diesel station soils. J Basic Microbiol 42:284-91 CrossRef
  8. Shields MS, Reagin MJ, Gerger RR, Campbell R, Somerville C (1995) TOM, a new aromatic degradative plasmid from / Burkholderia (Pseudomonas) cepacia G4. Appl Environ Microbiol 61:1352-356
  9. Taghavi S, Barac T, Greenberg B, Borremans B, Vangronsveld J, van der Lelie D (2005) Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene. Appl Environ Microbiol 71:8500-505 CrossRef
  10. Tiirola MA, M?nnist? MK, Puhakka JA, Kulomaa MS (2002) Evidence for natural horizontal transfer of the pcpB gene in the evolution of polychlorophenol-degrading Sphingomonads. Appl Environ Microbiol 68:4495-501 CrossRef
  11. van Elsas JD, Bailey MJ (2002) The ecology of transfer of mobile genetic elements. FEMS Microbiol Ecol 42:187-97 CrossRef
  12. Wang Y, Xiao M, Geng X, Liu J, Chen J (2007) Horizontal transfer of genetic determinants for degradation of phenol between the bacteria living in plant and its rhizosphere. Appl Microbiol Biotechnol 77:733-39 CrossRef
  13. Wang Y, Li H, Zhao W, He X, Chen J, Geng X, Xiao M (2010) Induction of toluene degradation and growth promotion in corn and wheat by horizontal gene transfer within endophytic bacteria. Soil Biol Biochem 42:1051-057 CrossRef
  14. Wang Y, Song J, Zhao W, He X, Chen J, Xiao M (2011) In situ degradation of phenol and promotion of plant growth in contaminated environments by a single / Pseudomonas aeruginosa strain. J Hazard Mater 192:354-60
  15. Yang L, Wang Y, Song J, Zhao W, He X, Chen J, Xiao M (2011) Promotion of plant growth and in situ degradation of phenol by an engineered / Pseudomonas fluorescens strain in different contaminated environments. Soil Biol Biochem 43:915-22 CrossRef
  
• 作者单位：Heping Wei (1)
  Yujing Wang (2)
  Chenhao Zhou (2)
  Qiuyue Jiang (2)
  Xiaoli He (2)
  Jun Chen (2)
  Ming Xiao (2)
  
  1. School of Life Science, Anqing Normal University, Anqing, 246011, China
  2. Biology Department, Development Center of Plant Germplasm, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People’s Republic China
  
• ISSN：1869-2044

文摘

Towards efficient application of horizontal gene transfer (HGT) techniques to soil remediation and agronomic production, transfer of plasmids involved in phenol degradation from a rhizobacterium to single and multiple recipients was investigated in soil. Regardless of single or multiple recipients, all transfer frequencies in soil with corn, wheat and tomato seedlings were significantly higher than those in soil without plants, indicating that plants can promote transfer of degradative plasmids among rhizobacteria. By and large, plant type had a low influence on transfer rate of degradative plasmid among rhizobacteria. The transfer of the degradative plasmid from the rhizobacterium to a mixture of 4- types of recipient produced higher transfer frequencies, phenol biodegradation and plant growth promotion than transfer to a single recipient or to 2- types of recipient. In most cases, the increase in transfer frequency correlated with promotion of plant growth and a decrease in phenol content. These results indicate that the presence of multiple strains as recipients significantly increases the overall transfer frequency and leads to the promotion of soil remediation and plant growth, which is important for application of HGT techniques to soil remediation and agronomic crop production.