Pc4, a putative movement protein of Rice stripe virus , interacts with a type I DnaJ protein and a small Hsp of rice

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• 作者：Lianming Lu ; Zhenguo Du ; Meiling Qin ; Ping Wang ; Hanhong Lan ; Xiaoqing Niu ; Dongsheng Jia ; Liyan Xie ; Qiying Lin and Lianhui Xie ; et al.
• 关键词：Rice stripe virus ; DnaJ protein ; Small Hsp ; Interaction
• 刊名：Virus Genes
• 出版年：2009
• 出版时间：April, 2009
• 年：2009
• 卷：38
• 期：2
• 页码：320-327
• 全文大小：471.7 KB

文摘

Rice stripe virus (RSV) infects rice and causes great yield reduction in some Asian countries. In this study, rice cDNA library was screened by a Gal4-based yeast two-hybrid system using pc4, a putative movement protein of RSV, as the bait. A number of positive colonies were identified and sequence analysis revealed that they might correspond to ten independent proteins. Two of them were selected and further characterized. The two proteins were a J protein and a small Hsp, respectively. Interactions between Pc4 and the two proteins were confirmed using coimmunoprecipitation. Implications of the findings that pc4 interacted with two chaperone proteins were discussed.