The influence of macro- and microelements in seminal plasma on diluted boar sperm quality
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- 作者：Maja Zakošek Pipan ; Janko Mrkun ; Breda Jakovac Strajn…
- 关键词：Macroelements ; Microelements ; Boar semen ; Liquid storage ; Sperm quality
- 刊名：Acta Veterinaria Scandinavica
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：59
- 期：1
- 全文大小：906KB
- 刊物主题：Veterinary Medicine/Veterinary Science; Animal Anatomy / Morphology / Histology;
- 出版者：BioMed Central
- ISSN：1751-0147
- 卷排序：59
文摘
BackgroundGrowing evidence indicates that macro- and microelements in the seminal plasma of humans and various domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress. In the present study, we therefore determined the concentrations of macro- and microelements in fresh boar seminal plasma and their relation to sperm quality parameters after 3 days of liquid storage was assessed. Twenty ejaculates from eight boars were collected, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability, mitochondrial membrane potential and DNA fragmentation were determined on the day of collection (day 0) and day 3 (72 h) of storage at 15–17 °C. Seminal plasma was separated and the concentrations of macroelements (Na, K, Ca, and Mg) and microelements (Cu, Fe, Zn and Se) were determined.