文摘
The rare N-unsubstituted glucosamine (GlcNH3 +) residues in heparan sulfate (HS) have important biological and pathophysiological roles. Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, GlcNH3 + - containing oligosaccharides from HS, would greatly contribute to investigating their natural role in HS. In this study, a hexasaccharide library that possess GlcNH3 + residues were prepared from the chemical modification of the fully sulfated dp6. Chemical reaction conditions were optimized to generate different pattern of GlcNH3 + - containing oligosaccharides, then the structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC/MS-ITTOF) analysis. EIC/MS and MS2 analysis showed different fragmentation patterns of dp6s with different GlcNH3 + residues. This provides a foundation for further identification and quantification of GlcNH3 + - oligosaccharides by mass spectrum analysis.