Interrelationship between lymphocytes and leptin in fat depots of obese mice revealed by changes in nutritional status

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• 作者：Karine Lolmède ; Alexia Zakaroff-Girard…
• 关键词：Fasting ; Refeeding ; Inflammation ; Macrophage ; Rag2??/li> High ; fat diet
• 刊名：Journal of Physiology and Biochemistry
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：71
• 期：3
• 页码：497-507
• 全文大小：1,266 KB
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• 作者单位：Karine Lolmède (1) (2)
  Alexia Zakaroff-Girard (1) (2)
  Cedric Dray (2) (3)
  Marie-Laure Renoud (1) (2)
  Danièle Daviaud (2) (3)
  Rémy Burcelin (2) (4)
  Max Lafontan (1) (2)
  Jean Galitzky (1) (2)
  Anne Bouloumié (1) (2)
  
  1. Inserm, UMR1048, Team 1, Institute of Metabolic and Cardiovascular Diseases, BP84225, 31432, Toulouse Cedex 4, France
  2. Paul Sabatier University, 118, Route de Narbonne, 31062, Toulouse Cedex 9, France
  3. Inserm, UMR1048, Team 3, Institute of Metabolic and Cardiovascular Diseases, BP84225, 31432, Toulouse Cedex 4, France
  4. Inserm, UMR1048, Team 2, Institute of Metabolic and Cardiovascular Diseases, BP84225, 31432, Toulouse Cedex 4, France
  
• 刊物主题：Biomedicine general; Human Physiology; Animal Physiology;
• 出版者：Springer Netherlands
• ISSN：1877-8755

文摘

The mechanisms underlying the relationships between nutritional status and immunity remain to be fully characterized. The present study was undertaken to analyze by flow cytometry, in the context of diet-induced obesity, the status of immune cells in subcutaneous, and epididymal fat depots in wild-type and immunodeficient Rag2??mice submitted to nutritional challenge, i.e., 48-h fasting and 1-week refeeding. In parallel, the responsiveness of mature adipocytes and immune cells in bone marrow, lymph node, and liver were also analyzed. The results show that fasting in obese wild-type mice induces a prominent lipolysis in epididymal AT and immunosuppression restricted to both subcutaneous and epididymal AT, characterized by reduced number of CD4+ T and B lymphocytes and M1/M2 macrophages associated with reduced leptin and increased FGF21 expression in mature adipocytes. One-week refeeding was sufficient to reverse the fasting-induced effects. Obese immunodeficient mice under nutritional challenge exhibited no changes in adipocyte leptin expression and no marked trafficking of AT macrophages or NK cells, while the fasted-induced upregulation of FGF21 expression was maintained as well as the lipolytic responses. The present results demonstrate that, in a context of diet-induced obesity, fasting-induced immunosuppression is restricted to fat depots in immunocompetent mice. Lack of adipocyte leptin regulation and fasting-induced immunosuppression in obese immunodeficient mice strongly suggests that lymphocytes are involved in the modulation of adipocyte leptin expression on one hand and on the other that leptin is involved in the immune changes in AT according to nutritional status.