Evidence of birth-and-death evolution of 5S rRNA gene in Channa species (Teleostei, Perciformes)

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• 作者：Anindya Sundar Barman ; Mamta Singh ; Rajeev Kumar Singh ; Kuldeep Kumar Lal
• 关键词：5S rDNA ; Channa ; NTS ; Evolution model
• 刊名：Genetica
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：144
• 期：6
• 页码：723-732
• 全文大小：1,670 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Life Sciences
  Animal Genetics and Genomics
  Plant Genetics and Genomics
  Human Genetics
  Microbial Genetics and Genomics
  
• 出版者：Springer Netherlands
• ISSN：1573-6857
• 卷排序：144

文摘

In higher eukaryotes, minor rDNA family codes for 5S rRNA that is arranged in tandem arrays and comprises of a highly conserved 120 bp long coding sequence with a variable non-transcribed spacer (NTS). Initially the 5S rDNA repeats are considered to be evolved by the process of concerted evolution. But some recent reports, including teleost fishes suggested that evolution of 5S rDNA repeat does not fit into the concerted evolution model and evolution of 5S rDNA family may be explained by a birth-and-death evolution model. In order to study the mode of evolution of 5S rDNA repeats in Perciformes fish species, nucleotide sequence and molecular organization of five species of genus Channa were analyzed in the present study. Molecular analyses revealed several variants of 5S rDNA repeats (four types of NTS) and networks created by a neighbor net algorithm for each type of sequences (I, II, III and IV) did not show a clear clustering in species specific manner. The stable secondary structure is predicted and upstream and downstream conserved regulatory elements were characterized. Sequence analyses also shown the presence of two putative pseudogenes in Channa marulius. Present study supported that 5S rDNA repeats in genus Channa were evolved under the process of birth-and-death.