Genome-wide mapping of matrix attachment regions in Drosophila melanogaster

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• 作者：Rashmi U Pathak (76)
  Arumugam Srinivasan (76)
  Rakesh K Mishra (76)
  
  76. Centre for Cellular and Molecular Biology ; Council of Scientific and Industrial Research ; Uppal Road ; Hyderabad ; 500 007 ; India
  
• 关键词：Nuclear matrix ; Matrix attachment regions ; Genome packaging
• 刊名：BMC Genomics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：15
• 期：1
• 全文大小：1,079 KB
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• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background Eukaryotic genome acquires functionality upon proper packaging within the nucleus. This process is facilitated by the structural framework of Nuclear Matrix, a nucleo-proteinaceous meshwork. Matrix Attachment Regions (MARs) in the genome serve as anchoring sites to this framework. Results Here we report direct sequencing of the MAR preparation from Drosophila melanogaster embryos and identify >7350 MARs. This amounts to ~2.5% of the fly genome and often coincide with AT rich non-coding regions. We find significant association of MARs with the origins of replication, transcription start sites, paused RNA Polymerase II sites and exons, but not introns, of highly expressed genes. We also identified sequence motifs and repeats that constitute MARs. Conclusion Our data reveal the contact points of genome to the nuclear architecture and provide a link between nuclear functions and genomic packaging.