Molecular Characterization of Commercial Varieties of Phyllanthus emblica Using RAPD and Nuclear rDNA SNPs

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• 作者：S. K. Singh ; P. R. Meghwal ; Rakesh Pathak
• 关键词：Aonla ; Genetic diversity ; RAPD ; Single nucleotide polymorphisms
• 刊名：Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：85
• 期：4
• 页码：971-978
• 全文大小：679 KB
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• 作者单位：S. K. Singh (1)
  P. R. Meghwal (1)
  Rakesh Pathak (1)
  
  1. Central Arid Zone Research Institute, Jodhpur, 342003, Rajasthan, India
  
• 刊物主题：Life Sciences, general; Behavioural Sciences; Plant Biochemistry; Nucleic Acid Chemistry;
• 出版者：Springer India
• ISSN：2250-1746

文摘

The Indian gooseberry, Phyllanthus emblica is an important minor fruit crop having commercial significance. Due to great tolerance to salinity and sodicity it is well adapted to arid conditions of Rajasthan. Genetic diversity studies of eight varieties Anand-2, Banarasi, Chakaiya, Francis, Krishna, Kanchan, NA7 and NA10 were carried out. The morpho-physiological characterization proved insufficient to distinguish studied varieties of P. emblica. Ten RAPD primers exhibited an average of 56.18 % polymorphism in banding pattern and 83.1 % PIC. They can be used as powerful markers to reveal genetic diversity in P. emblica. Present study validates the utility of ITS rDNA region being third generation molecular marker as a reliable indicator of phylogenetic interrelationships, especially for ITS regions as DNA barcode at higher levels can serve as an additional approach for identification and genetic cataloguing of P. emblica germplasm for crop improvement. Keywords Aonla Genetic diversity RAPD Single nucleotide polymorphisms