Identification of SSR Markers for Salt-tolerance in Rice Variety CSR10 by Selective Genotyping

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• 作者：Poonam Rana (1)
  Sunita Jain (2)
  Sheetal Yadav (1)
  Navinder Saini (1)
  R. K. Jain (1)
  
• 关键词：Basmati ; Oryza sativa ; rice ; SSR ; salt ; tolerance
• 刊名：Journal of Plant Biochemistry and Biotechnology
• 出版年：2009
• 出版时间：January 2009
• 年：2009
• 卷：18
• 期：1
• 页码：87-91
• 全文大小：97KB
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• 作者单位：Poonam Rana (1)
  Sunita Jain (2)
  Sheetal Yadav (1)
  Navinder Saini (1)
  R. K. Jain (1)
  
  1. Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar, 125 004, India
  2. CCS Haryana Agricultural University, Hisar, 125 004, India
  

文摘

A population of 171 F3 genotypes derived from a cross between CSR10 (salt tolerant, indica) and Taraori Basmati (HBC19) was evaluated for various salt-tolerance attributes at vegetative stage using a hydroponic culture system. Substantial variation was observed in F3 population for relative growth rate (range 0.065-.187), Na-K ratio (0.023-.376) and visual injury symptoms (score 1-). The mean individual score of CSR10 × HBC19 F3 plants ranged from 1.7 to 9.0 with mean value of 5.07. Seven of the F3 plants showed transgressive segregation for salt tolerance. F3 individuals at both extremes of the response distribution were selected and genotyped using 30 SSR markers displaying polymorphism between the two parental genotypes. As many as 18/30 SSR markers showed distorted segregation ratios among the 30 selected salt-tolerant and salt-sensitive CSR10 × HBC19 F3 plants. Linear regression analysis showed significant association of three markers (RM162 mapped on chromosome 6, and RM209 and RM287 on chromosome 11) with relative growth rate and two markers (RM212 on chromosome 1 and RM206 on chromosome 11) with Na-K ratio explaining 31.3% and 25.6% of phenotypic variation, respectively.