Association of systemic and intra-articular osteoclastogenic potential, pro-inflammatory mediators and disease activity with the form of inflammatory arthritis

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• 作者：Marina Iki? (1) (2)
  Zrinka Jaji? (3)
  Elvira Lazi? (4)
  Sanja Iv?evi? (1)
  Frane Grubi?i? (3)
  Ana Maru?i? (5)
  Nata?a Kova?i? (1)
  Danka Gr?evi? (1) (6)
  
• 关键词：Rheumatoid arthritis ; Psoriatic arthritis ; Osteoclasts ; Cytokines ; Inflammation ; Bone loss ; Peripheral blood ; Synovial fluid
• 刊名：International Orthopaedics
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：38
• 期：1
• 页码：183-192
• 全文大小：604 KB
• 作者单位：Marina Iki? (1) (2)
  Zrinka Jaji? (3)
  Elvira Lazi? (4)
  Sanja Iv?evi? (1)
  Frane Grubi?i? (3)
  Ana Maru?i? (5)
  Nata?a Kova?i? (1)
  Danka Gr?evi? (1) (6)
  
  1. Laboratory for Molecular Immunology, University of Zagreb School of Medicine, Salata 12, Zagreb, 10000, Croatia
  2. Department of Clinical Immunology and Rheumatology, University Hospital “Holy Ghost- University of Zagreb School of Medicine, Sveti Duh 64, Zagreb, 10000, Croatia
  3. Department of Rheumatology, Physical Medicine and Rehabilitation, Clinical Hospital Center “Sisters of Mercy- University of Zagreb School of Medicine, Vinogradska cesta 29, Zagreb, 10000, Croatia
  4. Department of Dermatology and Venereology, General Hospital “Dr. Ivo Pedi?i?- J.J.Strossmayera 59, Sisak, 44000, Croatia
  5. Department of Research in Biomedicine and Health, University of Split School of Medicine, Soltanska 2, Split, 21000, Croatia
  6. Department of Physiology and Immunology, University of Zagreb School of Medicine, Salata 3b, Zagreb, 10000, Croatia
  
• ISSN：1432-5195

文摘

Purpose We aimed to assess osteoclastogenic potential of peripheral blood mononuclear cells (PBMC) and synovial fluid-derived mononuclear cells (SFMC) in different forms of arthritis and to correlate it with inflammatory mediators within intra-articular and circulatory compartments. Methods Paired PBMC and SFMC samples of patients with rheumatoid arthritis (RA; n--0) and psoriatic arthritis (PsA; n--0), and PBMC of healthy controls were cultured to assess osteoclastogenic potential by the number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts (OCs) and expression of OC-related genes (receptor activator of nuclear factor-κΒ (RANK), cFMS, and TRAP). Osteoclastogenesis was correlated with the arthritis-related inflammatory indicators in serum and synovial fluid (SF). Results Number of OCs differentiated from PBMC was significantly higher in RA and PsA compared with control, with RA having more OCs compared with PsA. There was no difference in SFMC OC number between arthritic patients, but RANK expression in OCs differentiated from SFMC was higher in PsA compared with RA. SF of PsA patients more potently induced OC differentiation from control CD3-CD19-CD56-CD11b+CD115+ PBMC compared with RA, paralleled with higher RANK-ligand expression in PsA SFMC. Positive correlations of OC number with erythrocyte sedimentation rate, serum level of CCL2, and PBMC gene expression of interleukin-18 and Fas-ligand were observed. Conclusion Osteoclastogenic potential is systemically enhanced in patients with RA, paralleled by disordered systemic and local expression of proinflammatory mediators, whereas PsA involves specific deregulation in RANKL/RANK axis. Our study reveals arthritis-specific mediators associated with the form of arthritis, indicating clinical relevance for diagnosis and treatment.