Clonality testing of cutaneous lymphoid infiltrates: practicalities, pitfalls and potential uses

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• 作者：Anne M. Sproul (1)
  John R. Goodlad (2)
  
• 关键词：Polymerase chain reaction ; Skin ; Cutaneous infiltrates ; Clonality ; Reactive ; Neoplastic
• 刊名：Journal of Hematopathology
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：5
• 期：1-2
• 页码：69-82
• 全文大小：634KB
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• 作者单位：Anne M. Sproul (1)
  John R. Goodlad (2)
  
  1. Department of Haematology, Western General Hospital, Edinburgh, EH4 2XU, UK
  2. Department of Pathology, Western General Hospital and University of Edinburgh, Edinburgh, Crewe Road, EH4 2XU, UK
  

文摘

PCR is the method of choice for assessing antigen receptor gene rearrangement in suspect cutaneous infiltrates. It is a powerful and robust technique, but not without certain limitations and pitfalls. Of particular importance in the analysis of lymphoproliferations in the skin are the quality of DNA obtainable, an understanding of how primer choice may influence sensitivity and issues surrounding pseudoclonality. When interpreting results, cognisance must also be taken of the well-documented fact that monoclonal T-cell receptor and immunoglobulin gene rearrangements are not infrequently encountered in benign reactive cutaneous disorders. We discuss these issues in detail herein, and outline the situations in which we believe clonality testing may add value to assessing the biological nature of a cutaneous lymphoid infiltrate. Lastly, we emphasize the importance of interpreting any results derived from PCR assays in the context of the clinical, histological and immunophenotypic findings.