Spectroscopic and computational characterization of laccases and their substrate radical intermediates
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- 作者：Rebecca Pogni (1)
Maria Camilla Baratto (1)
Adalgisa Sinicropi (1)
Riccardo Basosi (1) - 关键词：Multicopper oxidases ; Copper catalytic site ; Laccase鈥檚 catalytic mechanism ; Substrate oxidation ; Paramagnetic species ; Radical intermediates ; EPR spectroscopy ; DFT calculations
- 刊名：Cellular and Molecular Life Sciences (CMLS)
- 出版年：2015
- 出版时间：March 2015
- 年：2015
- 卷：72
- 期：5
- 页码：885-896
- 全文大小：1,614 KB
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Maria Camilla Baratto (1)
Adalgisa Sinicropi (1)
Riccardo Basosi (1)
1. Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via A Moro 2, 53100, Siena, Italy - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Cell Biology
Biomedicine
Life Sciences
Biochemistry - 出版者：Birkh盲user Basel
- ISSN：1420-9071
文摘
Laccases are multicopper oxidases which oxidize a wide variety of aromatic compounds with the concomitant reduction of oxygen to water as by-product. Due to their high stability and biochemical versatility, laccases are key enzymes to be used as eco-friendly biocatalyst in biotechnological applications. The presence of copper paramagnetic species in the catalytic site paired with the substrate radical species produced in the catalytic cycle makes laccases particularly attractive to be studied by spectroscopic approaches. In this review, the potentiality of a combined multifrequency electron paramagnetic spectroscopy /computational approach to gain information on the nature of the catalytic site and radical species is presented. The knowledge at molecular level of the enzyme oxidative process can be of great help to model new enzymes with increased efficiency and robustness.