Folic-acid-conjugated pullulan/poly(DL-lactide-co-glycolide) graft copolymer nanoparticles for folate-receptor-mediated drug delivery

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• 作者：Sang Joon Lee (1)
  Yong-Ho Shim (2)
  Jong-Suk Oh (3)
  Young-Il Jeong (3)
  In-Kyu Park (1)
  Hyun Chul Lee (3)
  
  1. Department of Biomedical Sciences ; Chonnam National University Medical School ; Gwangju ; 501-746 ; Korea
  2. Biomedical Research Institute ; Pusan National University Hospital ; Pusan ; 602-739 ; Republic of Korea
  3. Department of Microbiology ; Chonnam National University Medical School ; Gwangju ; 501-746 ; Korea
  
• 关键词：Folate receptor ; Pullulan ; Nanoparticles ; KB cells ; Drug targeting
• 刊名：Nanoscale Research Letters
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：10
• 期：1
• 全文大小：1,646 KB
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• 刊物主题：Nanotechnology; Nanotechnology and Microengineering; Nanoscale Science and Technology; Nanochemistry; Molecular Medicine;
• 出版者：Springer US
• ISSN：1556-276X

文摘

Background Nanoparticles have been extensively investigated for targeted delivery of anticancer drugs. Since the folate receptor is universally over-expressed on the tumor cell membrane, folic acid is often used to modify the fate of nanoparticles in biologicals. Methods To fabricate targetable nanoparticles, folic acid was conjugated to a pullulan backbone and poly(DL-lactide-co-glycolide) (PLGA) (abbreviated as FAPuLG) was conjugated. KB cells and NIH3T3-cell-bearing mice were prepared to prove folate receptor targeting of FAPuLG nanoparticles. Results and discussion Nanoparticles of FAPuLG copolymer that self-assembled in water were small with diameters Doxorubicin (DOX) as a model drug was incorporated into the FAPuLG nanoparticles that were used to treat folate receptor over-expressing KB human carcinoma cells. Fluorescence microscopy revealed that DOX-incorporated FAPuLG nanoparticles induced strong red fluorescence in the KB cells in the absence of folic acid. However, fluorescence intensity was decreased by blocking folate receptors. Antitumor activity of FAPuLG nanoparticles against KB cells in vitro was also decreased by blocking folate receptors. In animal study using near-infrared dye-conjugated FAPuLG nanoparticles, fluorescence intensity was significantly higher at KB solid tumor than that of NIH3T3. Conclusions The results indicate that FAPuLG nanoparticles can target the folate receptor of tumor cells. FAPuLG nanoparticles are a promising candidate for active targeting of anticancer agents.