Superparamagnetic poly(methyl methacrylate) nanoparticles surface modified with folic acid presenting cell uptake mediated by endocytosis
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- 作者：Paulo Emilio Feuser ; Amanda Virtuoso Jacques…
- 关键词：Superparamagnetic ; Nanoparticles ; Poly(methyl methacrylate) ; Folic acid ; Cell uptake ; Targetted drugs ; Nanomedicine
- 刊名：Journal of Nanoparticle Research
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：18
- 期：4
- 全文大小：2,020 KB
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Amanda Virtuoso Jacques (2)
Juan Marcelo Carpio Arévalo (3)
Maria Eliane Merlin Rocha (3)
Maria Claudia dos Santos-Silva (2)
Claudia Sayer (1)
Pedro H. Hermes de Araújo (1)
1. Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Florianopolis, Brazil
2. Department of Clinical Analyses, Federal University of Santa Catarina, Florianopolis, Brazil
3. Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Nanotechnology
Inorganic Chemistry
Characterization and Evaluation Materials
Physical Chemistry
Applied Optics, Optoelectronics and Optical Devices - 出版者：Springer Netherlands
- ISSN：1572-896X
文摘
The encapsulation of superparamagnetic nanoparticles (MNPs) in polymeric nanoparticles (NPs) with modified surfaces can improve targeted delivery and induce cell death by hyperthermia. The goals of this study were to synthesize and characterize surface modified superparamagnetic poly(methyl methacrylate) with folic acid (FA) prepared by miniemulsion polymerization (MNPsPMMA-FA) and to evaluate their in vitro cytotoxicity and cellular uptake in non-tumor cells, murine fibroblast (L929) cells and tumor cells that overexpressed folate receptor (FR) β, and chronic myeloid leukemia cells in blast crisis (K562). Lastly, hemolysis assays were performed on human red blood cells. MNPsPMMA-FA presented an average mean diameter of 135 nm and a saturation magnetization (Ms) value of 37 emu/g of iron oxide, as well as superparamagnetic behavior. The MNPsPMMA-FA did not present cytotoxicity in L929 and K562 cells. Cellular uptake assays showed a higher uptake of MNPsPMMA-FA than MNPsPMMA in K562 cells when incubated at 37 °C. On the other hand, MNPsPMMA-FA showed a low uptake when endocytosis mechanisms were blocked at low temperature (4 °C), suggesting that the MNPsPMMA-FA uptake was mediated by endocytosis. High concentrations of MNPsPMMA-FA showed hemocompatibility when incubated for 24 h in human red blood cells. Therefore, our results suggest that these carrier systems can be an excellent alternative in targeted drug delivery via FR.