Folic acid modified electrospun poly(vinyl alcohol)/polyethyleneimine nanofibers for cancer cell capture applications

详细信息    查看全文

• 作者：Zhang-yu Fan ; Yi-li Zhao ; Xiao-yue Zhu 朱晓玥 ; Yu Luo…
• 刊名：Chinese Journal of Polymer Science
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：34
• 期：6
• 页码：755-765
• 全文大小：1,883 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Condensed Matter Physics
  Industrial Chemistry and Chemical Engineering
  Polymer Sciences
  Characterization and Evaluation of Materials
  
• 出版者：Chinese Chemical Society and Institute of Chemistry, CAS, co-published with Springer
• ISSN：1439-6203
• 卷排序：34

文摘

Capture and detection of metastatic cancer cells are crucial for diagnosis and treatment of malignant neoplasm. Here, we report the use of folic acid (FA) modified electrospun poly(vinyl alcohol) (PVA)/polyethyleneimine (PEI) nanofibers for cancer cell capture applications. Electrospun PVA/PEI nanofibers crosslinked by glutaraldehyde vapor were modified with FA via a poly(ethylene glycol) (PEG) spacer, followed by acetylation of the fiber surface PEI amines. The formed FA-modified nanofibers were well characterized. The morphology of the electrospun PVA/PEI nanofibers is smooth and uniform despite the surface modification. In addition, the FA-modified nanofibers display good hemocompatibility as confirmed by hemolysis assay. Importantly, the developed FA-modified nanofibers are able to specifically capture cancer cells overexpressing FA receptors, which were validated by quantitative cell counting assay and qualitative confocal microscopy analysis. The developed FA-modified PVA/PEI nanofibers may be used for capturing circulating tumor cells for cancer diagnosis applications.KeywordsPoly(vinyl alcohol)PolyethyleneimineElectrospun nanofibersFolic acidCancer cell capture