Folic acid-conjugated GdPO₄:Tb³⁺@SiO₂ Nanoprobe for folate receptor-targeted optical and magnetic resonance bi-modal imaging

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• 作者：Xianzhu Xu ; Xiaoying Zhang ; Yanli Wu
• 关键词：Nanoprobe ; Dual ; modality imaging ; GdPO4 ; Tb3+@SiO2 ; FA nanorods ; Optical imaging ; Magnetic resonance imaging
• 刊名：Journal of Nanoparticle Research
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：18
• 期：11
• 全文大小：5,642 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nanotechnology
  Inorganic Chemistry
  Characterization and Evaluation Materials
  Physical Chemistry
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer Netherlands
• ISSN：1572-896X
• 卷排序：18

文摘

Both fluorescent and magnetic nanoprobes have great potential applications for diagnostics and therapy. In the present work, a folic acid-conjugated and silica-modified GdPO₄:Tb3+ (GdPO₄:Tb3+@SiO₂-FA) dual nanoprobe was strategically designed and synthesized for the targeted dual-modality optical and magnetic resonance (MR) imaging via a facile aqueous method. Their structural, optical, and magnetic properties were determined using transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR), ultraviolet-visible spectra (UV-Vis), photoluminescence (PL), and superconducting quantum interference device (SQUID). These results indicated that GdPO₄:Tb3+@SiO₂-FA were uniform monodisperse core-shell structured nanorods (NRs) with an average length of ~200 nm and an average width of ~25 nm. The paramagnetic property of the synthesized GdPO₄:Tb3+@SiO₂-FA NRs was confirmed with its linear hysteresis plot (M-H). In addition, the NRs displayed an obvious T₁-weighted effect and thus it could potentially serve as a T₁-positive contrast agent. The NRs emitted green lights due to the 5D₄ → ^{7F₅ transition of the Tb3+. The in vitro assays with NCI-H460 lung cancer cells and human embryonic kidney cell line 293T cells indicated that the GdPO₄:Tb3+@SiO₂-FA nanoprobe could specifically bind the cells bearing folate receptors (FR). The MTT assay of the NRs revealed that its cytotoxicity was very low. Further in vivo MRI experiments distinctively depict enhanced anatomical features in a xenograft tumor. These results suggest that the GdPO₄:Tb3+@SiO₂-FA NPs have excellent imaging and cell-targeting abilities for the folate receptor-targeted dual-modality optical and MR imaging and can be potentially used as the nanoprobe for bioimaging.}