Activity concentrations of 90Sr in samples of wild boar bones hunted in different regions of Slovakia were determined. Molecular recognition technology product IBC´s AnaLig®Sr-01 and tributyl phosphate were used for strontium determination. From 7 to 10 g of bone ash was used for 90Sr analysis. Both separation methods were statistical tested and compared. The presented results were evaluated as correct for all experimental data for 90Sr determination in ash bone samples. Activity concentrations of 90Sr in bones were in the range of (4.5–69.0) Bq kg−1.