Different responses of soybean cyst nematode resistance between two RIL populations derived from Peking × 7605 under two ecological sites

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• 作者：YONGCHUN LI ; NA GUO ; JINMING ZHAO ; BIN ZHOU ; RAN XU ; HUI DING…
• 关键词：soybean cyst nematode ; resistance ; inheritance ; simple sequence repeat marker.
• 刊名：Journal of Genetics
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：95
• 期：4
• 页码：975-982
• 全文大小：
• 刊物主题：Life Sciences, general; Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Evolutionary Biology;
• 出版者：Springer India
• ISSN：0973-7731
• 卷排序：95

文摘

The soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is a plant-parasitic nematode that feeds on the roots of soybean and most economically devastating pathogen of soybean (Glycine max (L.) Merr.) worldwide. Host plant resistance is the most effective control method. To understand SCN resistance in different environments, two recombinant-inbred lines (RILs) populations NJ(RN)P7 (217 F _2:8:11 lines) and JN(RN)P7 (248 F _2:7:9 lines) were developed from the cross of the cultivars Peking × 7605 in Nanjing and Jinan, respectively, and examined in this study. Peking is resistant to SCN race 1 (HG types 2.5.7), while 7605 is highly susceptible. Chi-square test of frequency distribution of families’ female index (FI) showed that resistance to SCN was significantly different between NJ(RN)P7 and JN(RN)P7 populations. Three recessive genes conditioned the inheritance of resistance to SCN race 1 in both populations, but significant difference was detected for the mean of FI on two populations (DM = −16.68, P < 0.01). This indicated that natural selection may affect resistance to SCN. By analysing the variation of phenotype, the genetic structure of the two populations was determined to be different. The inheritance and variation of resistance were confirmed by simple sequence repeat (SSR) markers. For the two populations, 10 SSR markers showed polymorphism of resistant and susceptible DNA bulks. Some markers associated with the resistance of SCN races 1, 2, 3 and 5, and two markers, Satt163 and Satt309, reportedly related to rgh1 were detected both in NJ(RN)P7 and JN(RN)P7 populations. The results support the view that a disease acts as a selective force on plant resistance characteristics, which may alter the relative fitness of resistance alleles.