Rice pathogen Xanthomonas oryzae pv. oryzae employs inducible hrp-dependent XopF type III effector protein for its growth, pathogenicity and for suppression of PTI response to induce blight disease

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• 作者：Kalyan K. Mondal ; Geeta Verma ; Manju ; Alim Junaid…
• 关键词：Rice ; Xanthomonas ; Bacterial blight ; T3SS ; XopF
• 刊名：European Journal of Plant Pathology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：144
• 期：2
• 页码：311-323
• 全文大小：1,704 KB
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• 作者单位：Kalyan K. Mondal (1)
  Geeta Verma (1)
  Manju (1)
  Alim Junaid (1)
  Chandra Mani (1)
  
  1. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major disease of rice worldwide. Xoo secretes effector proteins (T3Es) directly into the rice cell through a hrp-encoded specialized type III secretion apparatus to induce blight. We examined the function of XopF, one of the conserved effectors in Xoo, using a null mutant developed through a PCR-based homologous recombination strategy. We studied inducible, hrp-dependent expression pattern of xopF. We confirmed that XopF is translocated in rice cytosol through T3SS using adenylate cyclase activity assay. XopF regulate the in planta Xoo growth and suppress PAMP-triggered immune (PTI) response in rice. Xoo wild but not mutant, Xoo 鈭?em class="EmphasisTypeItalic ">xopF produced intense blight lesions upon inoculation using leaf clipping method. Further, Xoo 鈭?em class="EmphasisTypeItalic ">xopF showed significant reduction in planta colonization relative to the wild strain. The relative expression analysis of PTI marker genes, PR10, OsWRKY13, OsRLCK16, and OsFLS2 indicated that these genes were up-regulated 1.5 to 5 fold upon challenge-inoculation with Xoo 鈭唜opF indicating the role of XopF in suppressing PTI in rice. Xoo 鈭唜opF mutant induced more callose deposition in infected rice leaves. XopF::EYFP fusion gene product was localized to the plasma membrane when transiently expressed in Nicotiana benthamiana as well as in living onion epidermal cells. Collectively, the present study shows that XopF repress basal PTI response in plants, and thus favours Xoo growth and pathogenicity in rice. Keywords Rice Xanthomonas Bacterial blight T3SS XopF