Preparation of PCL/silk fibroin/collagen electrospun fiber for urethral reconstruction

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• 作者：Gaijie Wei (1)
  Chao Li (1)
  Qiang Fu (1)
  Yuemin Xu (1)
  Hongbin Li (1)
  
  1. Department of Urology ; Affiliated Sixth People鈥檚 Hospital ; Shanghai Jiaotong University ; 600 Yishan Road ; Shanghai ; 200233 ; China
  
• 关键词：Urethral reconstruction ; Tissue engineering ; Polycaprolactone ; Silk fibroin ; Collagen ; Scaffold
• 刊名：International Urology and Nephrology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：47
• 期：1
• 页码：95-99
• 全文大小：701 KB
• 参考文献：1. Mathur RK, Nagar M, Mathur R, Khan F, Deshmukh C, Guru N (2014) Single-stage preputial skin flap urethroplasty for long-segment urethral strictures: evaluation and determinants of success. BJU Int 113(1):120鈥?26. doi:10.1111/bju.12361 CrossRef
  2. Kozinn SI, Harty NJ, Zinman L, Buckley JC (2013) Management of complex anterior urethral strictures with multistage buccal mucosa graft reconstruction. Urology 82(3):718鈥?22. doi:10.1016/j.urology.2013.03.081 CrossRef
  3. Xu YM, Sa YL, Fu Q, Zhang J, Xie H, Feng C (2013) A rationale for procedure selection to repair female urethral stricture associated with urethrovaginal fistulas. J Urol 189(1):176鈥?81. doi:10.1016/j.juro.2012.09.005 CrossRef
  4. Cui T, Terlecki R, Atala A (2014) Tissue engineering in urethral reconstruction. Arch Esp Urol 67(1):29鈥?4
  5. Li C, Xu YM, Song LJ, Fu Q, Cui L, Yin S (2008) Urethral reconstruction using oral keratinocyte seeded bladder acellular matrix grafts. J Urol 180(4):1538鈥?542. doi:10.1016/j.juro.2008.06.013 CrossRef
  6. Waxman SW, Morey AF (2006) Management of urethral strictures. Lancet 367(9520):1379鈥?380. doi:10.1016/s0140-6736(06)68592-9 CrossRef
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  8. Xu YM, Qiao Y, Sa YL, Wu DL, Zhang XR, Zhang J, Gu BJ, Jin SB (2007) Substitution urethroplasty of complex and long-segment urethral strictures: a rationale for procedure selection. Eur Urol 51(4):1093鈥?098; discussion 1098鈥?099. doi:10.1016/j.eururo.2006.11.039
  9. Kessler TM, Schreiter F, Kralidis G, Heitz M, Olianas R, Fisch M (2003) Long-term results of surgery for urethral stricture: a statistical analysis. J Urol 170(3):840鈥?44. doi:10.1097/01.ju.0000080842.99332.94 CrossRef
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  11. Li C, Xu Y, Song L, Fu Q, Cui L, Yin S (2008) Preliminary experimental study of tissue-engineered urethral reconstruction using oral keratinocytes seeded on BAMG. Urol Int 81(3):290鈥?95. doi:10.1159/000151406 CrossRef
  12. Ma Z, Kotaki M, Inai R, Ramakrishna S (2005) Potential of nanofiber matrix as tissue-engineering scaffolds. Tissue Eng 11(1鈥?):101鈥?09. doi:10.1089/ten.2005.11.101 CrossRef
  13. Courtney T, Sacks MS, Stankus J, Guan J, Wagner WR (2006) Design and analysis of tissue engineering scaffolds that mimic soft tissue mechanical anisotropy. Biomaterials 27(19):3631鈥?638. doi:10.1016/j.biomaterials.2006.02.024
  14. Williamson MR, Black R, Kielty C (2006) PCL-PU composite vascular scaffold production for vascular tissue engineering: attachment, proliferation and bioactivity of human vascular endothelial cells. Biomaterials 27(19):3608鈥?616. doi:10.1016/j.biomaterials.2006.02.025
  15. Jeong L, Yeo IS, Kim HN, Yoon YI, da Jang H, Jung SY, Min BM, Park WH (2009) Plasma-treated silk fibroin nanofibers for skin regeneration. Int J Biol Macromol 44(3):222鈥?28. doi:10.1016/j.ijbiomac.2008.12.008 CrossRef
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Nephrology
  Urology and Andrology
  
• 出版者：Springer Netherlands
• ISSN：1573-2584

文摘

Purpose To prepare polycaprolactone (PCL)/silk fibroin/collagen electrospun nanofiber scaffold and test its effects on growth and proliferation of oral mucosal epithelial cells. Methods Regenerated silk fibroin film, water-soluble collagen powder, and PCL, at mass ratios of 1:1:4, 1:1:8, and 1:1:10, were dissolved in hexafluoroisopropanol, and electrostatic spinning method was adopted to prepare PCL/silk fibroin/collagen electrospun nanofiber scaffold. In vitro cultured oral mucosal epithelial cells were inoculated on the material surface, MTT assay and scanning electron microscopy were adopted to study the growth and proliferation of oral mucosal epithelial cells on the material surface, and cell compatibility of PCL/silk fibroin/collagen electrospun nanofiber was evaluated. Results The result of MTT assay showed that oral mucosal epithelial cells were growing well on the PCL/silk fibroin/collagen electrospun nanofiber scaffold. Scanning electron microscopy showed that the prepared electrospun fiber was uniform in diameter and presented an interconnected porous net structure, and oral mucosal epithelial cells had a good growth form on the surface of the modified material. Conclusions PCL/silk fibroin/collagen electrospun nanofiber scaffold has appropriate pore size and porosity, is suitable for the growth of oral mucosal epithelial cells, has good cell compatibility, and is a good scaffold for tissue engineering urethral reconstruction.