Effects of Estradiol on Voltage-Gated Potassium Channels in Mouse Dorsal Root Ganglion Neurons
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- 作者：Juan Du (1) (2)
Qiang Wang (3)
Fang Hu (1)
Jun Wang (4)
Haixia Ding (4)
Rong Gao (4)
Hang Xiao (4)
Lin Wang (1) - 关键词：Estradiol ; Patch ; clamp recording ; Voltage ; gated potassium channels ; Dorsal root ganglion neurons
- 刊名：Journal of Membrane Biology
- 出版年：2014
- 出版时间：July 2014
- 年：2014
- 卷：247
- 期：7
- 页码：541-548
- 全文大小：
- 参考文献：1. Beckett EA, McCloskey C, O’Kane N, Sanders KM, Koh SD (2006) Effects of female steroid hormones on A-type K+ currents in murine colon. J Physiol 573(Pt 2):453-68 CrossRef
2. DeFazio RA, Moenter SM (2002) Estradiol feedback alters potassium currents and firing properties of gonadotropin-releasing hormone neurons. Mol Endocrinol 16(10):2255-265 CrossRef
3. Everill B, Kocsis JD (1999) Reduction in potassium currents in identified cutaneous afferent dorsal root ganglion neurons after axotomy. J Neurophysiol 82(2):700-08
4. Fatehi M, Kombian SB, Saleh TM (2005) 17beta-estradiol inhibits outward potassium currents recorded in rat parabrachial nucleus cells in vitro. Neuroscience 135(4):1075-086 CrossRef
5. Gintzler AR, Liu NJ (2012) Importance of sex to pain and its amelioration; relevance of spinal estrogens and its membrane receptors. Front Neuroendocrinol 33(4):412-24 CrossRef
6. Lee DY, Chai YG, Lee EB, Kim KW, Nah SY, Oh TH, Rhim H (2002) 17Beta-estradiol inhibits high-voltage-activated calcium channel currents in rat sensory neurons via a non-genomic mechanism. Life Sci 70(17):2047-059 CrossRef
7. Liu L, Simon SA (2003) Modulation of / I / A currents by capsaicin in rat trigeminal ganglion neurons. J Neurophysiol 89(3):1387-401 CrossRef
8. Liu H, Jin MW, Xiang JZ, Huang Y, Sun HY, Chiu SW, Lau CP, Li GR (2007) Raloxifene inhibits transient outward and ultra-rapid delayed rectifier potassium currents in human atrial myocytes. Eur J Pharmacol 563(1-):61-8 CrossRef
9. Lu Y, Jiang Q, Yu LH, Lu ZY, Meng SP, Su DF, Burnstock G, Ma B (2013) 17beta-Estradiol rapidly attenuates P2X3 receptor-mediated peripheral pain signal transduction via ERalpha and GPR30. Endocrinology 154(7):2421-433 CrossRef
10. McRoberts JA, Li J, Ennes HS, Mayer EA (2007) Sex-dependent differences in the activity and modulation of / N-methyl-d -aspartic acid receptors in rat dorsal root ganglia neurons. Neuroscience 148(4):1015-020 CrossRef
11. Micevych PE, Dewing P (2011) Membrane-initiated estradiol signaling regulating sexual receptivity. Front Endocrinol (Lausanne) 2:26
12. Nie H, Yu WJ, Li XY, Yuan CH, Pang YP, Li CY, Han YF, Li ZW (2007) Inhibition by bis(7)-tacrine of native delayed rectifier and KV 1.2 encoded potassium channels. Neurosci Lett 412(2):108-13 CrossRef
13. Stewart T, Beyak MJ, Vanner S (2003) Ileitis modulates potassium and sodium currents in guinea pig dorsal root ganglia sensory neurons. J Physiol 552(Pt 3):797-07 CrossRef
14. Takanami K, Sakamoto H, Matsuda K, Hosokawa K, Nishi M, Prossnitz ER, Kawata M (2010) Expression of G protein-coupled receptor 30 in the spinal somatosensory system. Brain Res 1310:17-8 CrossRef
15. Takeda M, Tsuboi Y, Kitagawa J, Nakagawa K, Iwata K, Matsumoto S (2011) Potassium channels as a potential therapeutic target for trigeminal neuropathic and inflammatory pain. Mol Pain 7:5 CrossRef
16. Taleghany N, Sarajari S, DonCarlos LL, Gollapudi L, Oblinger MM (1999) Differential expression of estrogen receptor alpha and beta in rat dorsal root ganglion neurons. J Neurosci Res 57(5):603-15 CrossRef
17. Vasudevan N, Pfaff DW (2008) Non-genomic actions of estrogens and their interaction with genomic actions in the brain. Front Neuroendocrinol 29(2):238-57 CrossRef
18. Vydyanathan A, Wu ZZ, Chen SR, Pan HL (2005) A-type voltage-gated K+ currents influence firing properties of isolectin B4-positive but not isolectin B4-negative primary sensory neurons. J Neurophysiol 93(6):3401-409 CrossRef
19. Wang Q, Cao J, Zhu Q, Luan C, Chen X, Yi X, Ding H, Chen J, Cheng J, Xiao H (2011) Inhibition of voltage-gated sodium channels by bisphenol A in mouse dorsal root ganglion neurons. Brain Res 1378:1- CrossRef
20. Wang Q, Cao J, Hu F, Lu R, Wang J, Ding H, Gao R, Xiao H (2013a) Effects of estradiol on voltage-gated sodium channels in mouse dorsal root ganglion neurons. Brain Res 1512:1- CrossRef
21. Wang Q, Ye Q, Lu R, Cao J, Wang J, Ding H, Gao R, Xiao H (2013b) Effects of estradiol on high-voltage-activated Ca2+ channels in cultured rat cortical neurons. Endocr Res 39(1):44-9
22. Watson CS, Alyea RA, Jeng YJ, Kochukov MY (2007) Nongenomic actions of low concentration estrogens and xenoestrogens on multiple tissues. Mol Cell Endocrinol 274(1-):1- CrossRef
23. Wong CM, Tsang SY, Yao X, Chan FL, Huang Y (2008) Differential effects of estrogen and progesterone on potassium channels expressed in Xenopus oocytes. Steroids 73(3):272-79 CrossRef
24. Xu S, Cheng Y, Keast JR, Osborne PB (2008) 17beta-estradiol activates estrogen receptor beta-signalling and inhibits transient receptor potential vanilloid receptor 1 activation by capsaicin in adult rat nociceptor neurons. Endocrinology 149(11):5540-548 CrossRef
25. Zhang C, Kelly MJ, Ronnekleiv OK (2010) 17beta-estradiol rapidly increases ATP-sensitive potassium channel activity in gonadotropin-releasing hormone neurons [corrected] via a protein kinase signaling pathway. Endocrinology 151(9):4477-484 CrossRef
26. Zhang Y, Lu N, Zhao ZQ, Zhang YQ (2012) Involvement of estrogen in rapid pain modulation in the rat spinal cord. Neurochem Res 37(12):2697-705 CrossRef - 作者单位：Juan Du (1) (2)
Qiang Wang (3)
Fang Hu (1)
Jun Wang (4)
Haixia Ding (4)
Rong Gao (4)
Hang Xiao (4)
Lin Wang (1)
1. Institute of Stomatology, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China
2. Department of Stomotology, Huai’an First People’s Hospital, 6 Beijing Road West, Huai’an, 223300, China
3. Department of Preventive Medicine, School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, China
4. The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 140 Hanzhong Road, Nanjing, 210029, China - ISSN：1432-1424
文摘
Voltage-gated potassium channels are regulators of membrane potentials, action potential shape, firing adaptation, and neuronal excitability in excitable tissues including in the primary sensory neurons of dorsal root ganglion (DRG). In this study, using the whole-cell patch-clamp technique, the effect of estradiol (E2) on voltage-gated total outward potassium currents, the component currents transient “A-type-current (I A) currents, and “delayed rectifier type-(I KDR) currents in isolated mouse DRG neurons was examined. We found that the extracellularly applied 17β-E2 inhibited voltage-gated total outward potassium currents; the effects were rapid, reversible, and concentration-dependent. Moreover, the membrane impermeable E2-BSA was as efficacious as 17β-E2, whereas 17α-E2 had no effect. 17β-E2-stimulated decrease in the potassium current was unaffected by treatment with ICI 182780 (classic estrogen receptor antagonist), actinomycin D (RNA synthesis inhibitor), or cycloheximide (protein synthesis inhibitor). We also found that I A and I KDR were decreased after 17β-E2 application. 17β-E2 significantly shifted the activation curve for I A and I KDR channels in the hyperpolarizing direction. In conclusion, our results demonstrate that E2 inhibited voltage-gated K+ channels in mouse DRG neurons through a membrane ER-activated non-genomic pathway.