Inward rectifier potassium current (I K1) and Kir2 composition of the zebrafish (Danio rerio) heart

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• 作者：Minna Hassinen ; Jaakko Haverinen…
• 关键词：Zebrafish ; Heart ; Inward rectifier potassium current ; Kir2 channel
• 刊名：Pfl篓鹿gers Archiv - European Journal of Physiology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：467
• 期：12
• 页码：2437-2446
• 全文大小：951 KB
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• 作者单位：Minna Hassinen (1)
  Jaakko Haverinen (1)
  Matt E. Hardy (2)
  Holly A. Shiels (2)
  Matti Vornanen (1)
  
  1. Department of Biology, University of Eastern Finland, P.O. Box 111, 80101, Joensuu, Finland
  2. Faculty of Life Sciences, University of Manchester, 46 Grafton Street, Manchester, M13 9NT, UK
  
• 刊物主题：Human Physiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1432-2013

文摘

Electrophysiological properties and molecular background of the zebrafish (Danio rerio) cardiac inward rectifier current (I_K1) were examined. Ventricular myocytes of zebrafish have a robust (?.7?±-.2 pA pF? at ?20 mV) strongly rectifying and Ba2+-sensitive (IC₅₀--.8 μM) I_K1. Transcripts of six Kir2 channels (drKir2.1a, drKir2.1b, drKir2.2a, drKir2.2b, drKir2.3, and drKir2.4) were expressed in the zebrafish heart. drKir2.4 and drKir2.2a were the dominant isoforms in both the ventricle (92.9?±-.5 and 6.3?±-.5 %) and the atrium (28.9?±-.9 and 64.7?±-.0 %). The remaining four channels comprised together less than 1 and 7 % of the total transcripts in ventricle and atrium, respectively. The four main gene products (drKir2.1a, drKir2.2a, drKir2.2b, drKir2.4) were cloned, sequenced, and expressed in HEK cells for electrophysiological characterization. drKir2.1a was the most weakly rectifying (passed more outward current) and drKir2.2b the most strongly rectifying (passed less outward current) channel, whilst drKir2.2a and drKir2.4 were intermediate between the two. In regard to sensitivity to Ba2+ block, drKir2.4 was the most sensitive (IC₅₀--.8 μM) and drKir2.1a the least sensitive channel (IC₅₀--32 μM). These findings indicate that the Kir2 isoform composition of the zebrafish heart markedly differs from that of mammalian hearts. Furthermore orthologous Kir2 channels (Kir2.1 and Kir2.4) of zebrafish and mammals show striking differences in Ba2+-sensitivity. Structural and functional differences needs to be taken into account when zebrafish is used as a model for human cardiac electrophysiology, cardiac diseases, and in screening cardioactive substances. Keywords Zebrafish Heart Inward rectifier potassium current Kir2 channel