Exogenously applied nitrate improves the photosynthetic performance and nitrogen metabolism in tomato (Solanum lycopersicum L. cv Pusa Rohini) under arsenic (V) toxicity
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- 作者：Ashish Agnihotri ; Chandra Shekhar Seth
- 关键词：Nitrate reductase ; Nitrite reductase ; Photosynthetic quantum yield ; Solanum lycopersicum L. ; Scanning electron microscope ; Nitric oxide
- 刊名：Physiology and Molecular Biology of Plants
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：22
- 期：3
- 页码：341-349
- 全文大小：1,245 KB
- 刊物主题：Plant Sciences; Plant Physiology; Biophysics and Biological Physics; Cell Biology;
- 出版者：Springer India
- ISSN：0974-0430
- 卷排序：22
文摘
Tomato (Solanum lycopersicum L.) being a widespread and most commonly consumed vegetable all over the world has an important economic value for its producers and related food industries. It is a serious matter of concern as its production is affected by arsenic present in soil. So, the present study, investigated the toxicity of As(V) on photosynthetic performance along with nitrogen metabolism and its alleviation by exogenous application of nitrate. Plants were grown under natural conditions using soil spiked with 25 mg and 20 mM, As(V) and nitrate, respectively. Our results revealed that plant growth indices, photosynthetic pigments, and other major photosynthetic parameters like net photosynthetic rate and maximum quantum efficiency (Fv/Fm) of photosystem II (PSII) were significantly (P ≤ 0.05) reduced under As(V) stress. However, nitrate application significantly (P ≤ 0.05) alleviated As(V) toxicity by improving the aforesaid plant responses and also restored the abnormal shape of guard cells. Nitrogen metabolism was assessed by studying the key nitrogen-metabolic enzymes. Exogenous nitrate revamped nitrogen metabolism through a major impact on activities of NR, NiR, GS and GOGAT enzymes and also enhanced the total nitrogen and NO content while malondialdehyde content, and membrane electrolytic leakage were remarkably reduced. Our study suggested that exogenous nitrate application could be considered as a cost effective approach in ameliorating As(V) toxicity.