Abstract To establish a sampling procedure for estimating the density of bamboo galls induced by Aiolomorphus rhopaloides Walker (Hymenoptera: Eurytomidae) in a stand of bamboo Phyllostachys heterocycla (Carr. Mitf.) (Monocotyledoneae: Gramineae), 5-12 bamboo culms were cut at random in late March of each year during 1998-2001 just before gall-maker emergence. The number of galls on the branches of each bamboo culm was counted. The spatial patterns of the galls on the culms in the stand and on the branches within each culm were measured by Iwao's patchiness regression. Galls were distributed contagiously both on culms and on branches. Current bamboo culms that emerged the previous summer did not require sampling because no galls were observed on them. Except for this, there was no difference in gall density on young and old culms, suggesting that it is not necessary to distinguish them. There were few (<4%) galls above 6 m height during the 4 years. There was no significant difference in gall density up to 4 m and above 4 m, suggesting that branches up to 4 m can be sampled with confidence. Kuno's two-stage sampling method at different precision levels showed that the number of culms to be sampled varied with gall density. For example, at mean density m = 1.0 per branch when the number of sampled branches per culm is 10, a total of 24 culms is required to estimate gall number at a precision level of D = 0.2, where D is the ratio of standard error to mean.