Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells

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• 作者：So Dam Jin ; Bo Ram Lee ; Young Sun Hwang…
• 关键词：Chicken ; Chicken vasa homologue ; Primordial germ cell ; Regulatory element ; siRNA ; mediated knockdown
• 刊名：Journal of Animal Science and Biotechnology
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：8
• 期：1
• 全文大小：2103KB
• 刊物主题：Agriculture; Biotechnology; Food Science; Animal Genetics and Genomics; Animal Physiology;
• 出版者：BioMed Central
• ISSN：2049-1891
• 卷排序：8

文摘

BackgroundPrimordial germ cells (PGCs), the precursors of functional gametes, have distinct characteristics and exhibit several unique molecular mechanisms to maintain pluripotency and germness in comparison to somatic cells. They express germ cell-specific RNA binding proteins (RBPs) by modulating tissue-specific cis- and trans-regulatory elements. Studies on gene structures of chicken vasa homologue (CVH), a chicken RNA binding protein, involved in temporal and spatial regulation are thus important not only for understanding the molecular mechanisms that regulate germ cell fate, but also for practical applications of primordial germ cells. However, very limited studies are available on regulatory elements that control germ cell-specific expression in chicken. Therefore, we investigated the intricate regulatory mechanism(s) that governs transcriptional control of CVH.