Effects of ferrous carbamoyl glycine on iron state and absorption in an iron-deficient rat model

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• 作者：Yuzhe Zhang ; Xiaoming Sun ; Chunyan Xie ; Xugang Shu…
• 关键词：Ferrous carbamoyl glycine ; Hepcidin ; IRP1/IRP2 ; SD rats ; FeSO4
• 刊名：Genes & Nutrition
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：10
• 期：6
• 全文大小：800 KB
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• 作者单位：Yuzhe Zhang (1)
  Xiaoming Sun (1)
  Chunyan Xie (1)
  Xugang Shu (1) (2) (3)
  Abimbola Oladele Oso (1) (4)
  Zheng Ruan (2)
  Ze-yuan Deng (2)
  Xin Wu (1) (2)
  Yulong Yin (1) (2)
  
  1. Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China
  2. State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang, 330031, China
  3. Guangzhou Tanke BIO-TECH Co. Ltd., Guangzhou, 510800, China
  4. Department of Animal Nutrition, College of Animal Science and Livestock Production, PMB 2240, Abeokuta, Nigeria
  
• 刊物主题：Human Genetics; Nutrition; Gene Function; Biomedicine general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1865-3499

文摘

An iron-deficient rat model was established and used to determine the effects of different iron sources on iron metabolism and absorption. Iron-deficient rats were assigned to one of three treatment groups, and their diet was supplemented with deionized water (control), Fe-CGly, or FeSO₄ for 8 days via intragastric administration. Blood samples were obtained for analysis of iron-related properties, and the small intestine and liver were removed for quantitative reverse transcription PCR of genes related to iron metabolism. The serum total iron-binding capacity (TIBC) levels of rats in Fe-CGly and FeSO₄ supplementation groups was lower (P < 0.05) than that of the rats in the control group. The rats in Fe-CGly group exhibited higher (P < 0.05) plasma Fe and ferritin levels and lower (P < 0.05) TIBC levels compared with the rats in FeSO₄ groups. The relative expression of liver hepcidin increased (P < 0.05) by tenfold and 80-fold in the Fe-CGly and FeSO₄ groups, respectively, whereas divalent metal transporter 1, duodenal cytochrome b, and ferroportin 1 expression decreased (P < 0.05) in the duodenum in both Fe-CGly and FeSO₄ group. A comparison between Fe-CGly and FeSO₄ group showed that iron regulatory protein 1 (IRP1) and iron regulatory protein (IRP2) expressions were reduced (P < 0.05) in rats administered FeSO₄ than in rats administered with Fe-Cgly. These results indicate that Fe-CGly rapidly improves the blood iron status and that IRP1 and IRP2 may play an important role in the intestinal absorption of Fe-CGly.